Results 91 to 100 of about 2,168,705 (292)
Phosphorus is an indispensable nutrient for nitrogen metabolism in soybeans. In this study, two P levels were established, 1 mg/L (low-P stress) and 31 mg/L (normal P, CK), by combining 15N labeling with real-time quantitative PCR and the UHPLC-MS/MS ...
Yubo Yao, Xinlei Liu
doaj +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Maize (Zea mays L.) is a globally significant crop with high economic and nutritional importance. Its productivity, however, relies heavily on nitrogen (N) inputs, often resulting in low nitrogen use efficiency (NUE).
Muhammad Faheem Jan +6 more
doaj +1 more source
O objetivo deste trabalho foi avaliar a resposta e a eficiência do uso de N, por cultivares de feijoeiro, em função do manejo do fertilizante nitrogenado em solo de várzea.
Alberto Baêta dos Santos +1 more
doaj +1 more source
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
In Gannan navel orange (Citrus sinensis) orchards—a typical sloped farmland ecosystem—selected native grasses outperform conventional green manure due to their stronger ecological adaptability and lower management requirements.
Wenqian Wang +10 more
doaj +1 more source
The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav +4 more
wiley +1 more source
Dryland winter wheat in the Loess Plateau is facing a yield reduction due to a shortage of soil moisture and delayed sowing time. The field experiment was conducted at Loess Plateau in Shanxi, China from 2012 to 2015, to study the effect of subsoiling ...
Yan Fei Liang +6 more
doaj +1 more source
MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima +8 more
wiley +1 more source
TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao +10 more
wiley +1 more source

