Results 31 to 40 of about 103,759 (256)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Evaluation of noise control in industrial buildings through simulations

Advances in Civil and Architectural Engineering
The most common detrimental effect of noise among workers in industrial facilities is permanent hearing loss. In the literature review, it was found that the combined effect of increasing the total sound absorption and noise barriers on noise control in ...
Okan Şimşek
doaj   +1 more source

Absorption spectroscopy at the ultimate quantum limit from single-photon states

New Journal of Physics, 2017
Absorption spectroscopy is routinely used to characterise chemical and biological samples. For the state-of-the-art in laser absorption spectroscopy, precision is theoretically limited by shot-noise due to the fundamental Poisson-distribution of photon ...
R Whittaker, C Erven, A Neville, M Berry, J L O’Brien, H Cable, J C F Matthews   +6 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Probing THz intersubband absorption using Johnson noise thermometry

Nanophotonics
We investigate the THz intersubband absorption behavior of a single 40-nm wide GaAs/AlGaAs square quantum well (QW) using Johnson noise thermometry. In our measurements, the Johnson noise associated with intersubband absorption is measured from the in ...
Yoo Changyun, Sherwin Mark S., West Kenneth W., Pfeiffer Loren N., Kawamura Jonathan H., Karasik Boris S.   +5 more
doaj   +1 more source

Application of Recycled Carpet Composite as a potential Noise Barrier in Infrastructure Applications

Recycling, 2019
In this work, recycling of post-consumer carpets into structural composites using a modified vacuum assisted resin transfer molding process has been demonstrated.
Kunal Mishra, Ranji K. Vaidyanathan
doaj   +1 more source

Experimental Analysis and Simulation of a Porous Absorbing Layer for Noise Barriers

Applied Sciences, 2023
Noise barriers are common noise mitigation measures usually implemented near roads or railways, with proven efficiency. This work presents a study of a porous concrete material incorporating expanded clay as aggregate, to be used on the sound-absorption ...
Laura Sousa, Luís Pereira, David Montes-González, Denilson Ramos, Paulo Amado-Mendes, Juan Miguel Barrigón-Morillas, Luís Godinho   +6 more
doaj   +1 more source