Results 101 to 110 of about 28,076,457 (358)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Identifying Noise And Vibration Of The Discharge Stage In The Rotary Compressor Based On Angle Domain Analysis Method [PDF]

open access: yes, 2012
In the noise and vibration test of the rotary compressor, the peak value of noise and the corresponding frequency can be identified according to the analysis of frequency spectrum.
Xu, Jia, Zhang, Rongting, Gu, Huanhuan
core   +1 more source

Matched subspace detection with hypothesis dependent noise power [PDF]

open access: yes, 2008
We consider the problem of detecting a subspace signal in white Gaussian noise when the noise power may be different under the null hypothesis—where it is assumed to be known—and the alternative hypothesis.
Cédric Richard   +8 more
core   +1 more source

A Summing Configuration based Low Noise Amplifier for MPI and MPS

open access: yesCurrent Directions in Biomedical Engineering, 2018
Magnetic particle imaging (MPI) is a novel tomographic imaging modality which uses static and dynamic magnetic fields to measure the magnetic response generated by superparamagnetic iron oxide nanoparticles (SPIONs).
Malhotra Ankit, Buzug Thorsten M.
doaj   +1 more source

Heavy-tailed prior distributions for sequence count data: removing the noise and preserving large differences

open access: yesbioRxiv, 2018
In RNA-seq differential expression analysis, investigators aim to detect genes with changes in expression across conditions, despite technical and biological variability. A common task is to accurately estimate the effect size. When the counts are low or
Anqi Zhu, J. Ibrahim, M. Love
semanticscholar   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

The calculation of noise from railway bridges and viaducts [PDF]

open access: yes, 2005
Pandrol Rail Fastenings Limited are a designer and manufacturer of railway rail-fastening systems. As an organisation they have the capability to reduce the noise impact of bridges using resilient track components. They also have a commercial interest in
Bewes, Oliver Guy
core  

EMD-based filtering (EMDF) of low-frequency noise for speech enhancement [PDF]

open access: yes, 2012
An Empirical Mode Decomposition based filtering (EMDF) approach is presented as a post-processing stage for speech enhancement. This method is particularly effective in low frequency noise environments.
Navin Chatlani   +4 more
core   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

open access: yesFEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya   +2 more
wiley   +1 more source

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