Results 81 to 90 of about 305,947 (290)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Impulse Shaping Method Based on Bayesian Deconvolution

Yuanzineng kexue jishu
A novel impulse shaping method based on Bayesian deconvolution as a means of enhancing the energy range of the energy spectrum was proposed. The initial step of this method entailed expressing the measured pulse signal as the convolution of the current ...
JIA Tonghui, LIU Yi, WANG Ming, MA Yingjie, WANG Bo
doaj   +1 more source

The design of optimum filters for quantizing a class of non bandlimited signals [PDF]

, 1998
We consider the efficient quantization of a class of non bandlimited signals, namely the class of discrete time signals that can be recovered from their decimated version.
Tuqan, Jamal, Vaidyanathan, P. P.
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

A SAR-Assisted Continuous-Time M-0 MASH Delta-Sigma Modulator With Digital-Domain Noise Leakage Shaping

IEEE Open Journal of Circuits and Systems
Delta–sigma ( $\Delta \Sigma $ ) modulators are widely used in high-resolution analog-to-digital converters (ADCs). With continued technology scaling, digital-intensive $\Delta \Sigma $ architectures are increasingly favored over traditional ...
Kent Edrian Lozada, Bo Gao, Raymond Mabilangan, Charlie Tahar, Kun-Woo Park, Young-Hun Moon, Kwan-Hoon Song, Seung-Tak Ryu   +7 more
doaj   +1 more source

Spectral broadening and shaping of nanosecond pulses: towards shaping of single photons from quantum emitters

, 2014
We experimentally demonstrate spectral broadening and shaping of exponentially-decaying nanosecond pulses via nonlinear mixing with a phase-modulated pump in a periodically-poled lithium niobate (PPLN) waveguide. A strong, 1550~nm pulse is imprinted with
Agha, Imad, Ates, Serkan, Sapienza, Luca, Srinivasan, Kartik   +3 more
core   +1 more source

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

A High-Dynamic-Range Switched-Capacitor Sigma-Delta ADC for Digital Micromechanical Vibration Gyroscopes

Micromachines, 2018
This paper presents a multi-stage noise shaping (MASH) switched-capacitor (SC) sigma-delta (ΣΔ) analog-to-digital converter (ADC) composed of an analog modulator with an on-chip noise cancellation logic and a reconfigurable digital decimator ...
Risheng Lv, Weiping Chen, Xiaowei Liu
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source