Results 111 to 120 of about 11,400,092 (255)

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

Asymmetric synthesis of fluorinated derivatives of aromatic and γ-branched amino acids via a chiral Ni(II) complex

Beilstein Journal of Organic Chemistry
Fluorinated amino acids are essential building blocks in the spheres of protein engineering and medicinal chemistry. In the last decades, a large number of different synthetic strategies have been developed to produce a large variety of fluorinated amino
Maurizio Iannuzzi, Thomas Hohmann, Michael Dyrks, Kilian Haoues, Katarzyna Salamon-Krokosz, Beate Koksch   +5 more
doaj   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Loss of FTO Antagonises Wnt Signaling and Leads to Developmental Defects Associated with Ciliopathies [PDF]

, 2014
Common intronic variants in the Human fat mass and obesity-associated gene (FTO) are found to be associated with an increased risk of obesity. Overexpression of FTO correlates with increased food intake and obesity, whilst loss-of-function results in ...
Rosa Maria Roccasecca (519728), Osborn Daniel P. S., Beales, PL, Daniel P. S. Osborn (519727), Fiona McMurray, Derek Stemple (183414), McMurray, F, Stemple Derek, Roccasecca, RM, Barroso, I, Philip L. Beales (492737), Osborn, DP, Inês Barroso (2144), Hernandez-Hernandez, V, Mukherjee, S, Derek Stemple, Cox, R, Philip L Beales, Victor Hernandez-Hernandez, Stemple, D, Sonia Christou-Savina (519731), Christou-Savina, S, Barroso Inês, Sriparna Mukherjee, Mukherjee Sriparna, Roccasecca Rosa Maria, Cox Roger, Sonia Christou-Savina, Hernandez-Hernandez Victor, Victor Hernandez-Hernandez (519729), Fiona McMurray (106290), Rosa Maria Roccasecca, Sriparna Mukherjee (519730), Beales Philip L., Daniel P S Osborn, Inês Barroso, McMurray Fiona, Osborn, DPS, Roger Cox, Christou-Savina Sonia, Roger Cox (73899)   +40 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

The Application of Cell-Free Protein Synthesis in Genetic Code Expansion for Post-translational Modifications

Frontiers in Pharmacology, 2019
The translation system is a sophisticated machinery that synthesizes proteins from 20 canonical amino acids. Recently, the repertoire of such composition has been expanded by the introduction of non-canonical amino acids (ncAAs) with the genetic code ...
Sumana Venkat, Hao Chen, Qinglei Gan, Chenguang Fan, Chenguang Fan   +4 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Non-canonical gender in African languages

, 2018
This chapter investigates interactions between gender and number, and between gender and evaluative morphology in eighty-four African languages. It argues that interactions of gender with other grammatical domains (e.g.
Yvonne Agbetsoamedo, Agbetsoamedo, Yvonne, Di Garbo, Francesca,, Francesca Di Garbo   +3 more
core   +1 more source

Threonine 348 regulates the subcellular localization of PTEN

FEBS Open Bio, EarlyView.
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley   +1 more source