Using cryoEM and cryoET to visualize membrane penetration of a non-enveloped virus [PDF]
STAR Protocols, 2022 Summary: Key to cell entry by non-enveloped viruses is virus-cell interactions at the cell or endosomal membrane. Here, we detail our protocols to capture such interactions between non-enveloped virus bluetongue virus (BTV) and vesicular membrane by ...
Xian Xia, Z. Hong Zhou
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Size-selective downstream processing of virus particles and non-enveloped virus-like particles [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Non-enveloped virus-like particles (VLPs) are versatile protein nanoparticles with great potential for biopharmaceutical applications. However, conventional protein downstream processing (DSP) and platform processes are often not easily applicable due to
Nils Hillebrandt, Jürgen Hubbuch
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EMC1-dependent stabilization drives membrane penetration of a partially destabilized non-enveloped virus [PDF]
eLife, 2016 Destabilization of a non-enveloped virus generates a membrane transport-competent viral particle. Here we probe polyomavirus SV40 endoplasmic reticulum (ER)-to-cytosol membrane transport, a decisive infection step where destabilization initiates this non-
Parikshit Bagchi +2 more
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Non-enveloped virus membrane penetration: New advances leading to new insights. [PDF]
PLoS Pathogens, 2022 Host cell membranes pose a particular challenge for non-enveloped viruses. Whereas enveloped viruses enter cells by fusing their lipid envelopes with the cellular membrane, non-enveloped viruses generally must (1) enter cells via endocytosis, then (2 ...
Madison L Pletan, Billy Tsai
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Enveloped and non-enveloped virus survival on microfiber towels [PDF]
PeerJ, 2023 Background Handwashing is an important intervention which can reduce indirect disease transmission, however soap and water for handwashing purposes is not available in some low-resource regions.
Claire E. Anderson +2 more
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Multiple conformations of trimeric spikes visualized on a non-enveloped virus. [PDF]
Nat Commun, 2022 Many viruses utilize trimeric spikes to gain entry into host cells. However, without in situ structures of these trimeric spikes, a full understanding of this dynamic and essential process of viral infections is not possible. Here we present four in situ
Zhang Y, Cui Y, Sun J, Zhou ZH.
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Functional refolding of the penetration protein on a non-enveloped virus. [PDF]
Nature, 2021 A non-enveloped virus requires a membrane lesion to deliver its genome into a target cell1. For rotaviruses, membrane perforation is a principal function of the viral outer-layer protein, VP42,3.
Herrmann T +7 more
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Structural Insight into Non-Enveloped Virus Binding to Glycosaminoglycan Receptors: A Review. [PDF]
Viruses, 2021 Viruses are infectious agents that hijack the host cell machinery in order to replicate and generate progeny. Viral infection is initiated by attachment to host cell receptors, and typical viral receptors are cell-surface-borne molecules such as proteins
Sorin MN, Kuhn J, Stasiak AC, Stehle T.
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Pathogen reduction in human plasma using an ultrashort pulsed laser. [PDF]
PLoS ONE, 2014 Pathogen reduction is a viable approach to ensure the continued safety of the blood supply against emerging pathogens. However, the currently licensed pathogen reduction techniques are ineffective against non-enveloped viruses such as hepatitis A virus ...
Shaw-Wei D Tsen +8 more
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Complexity and ultrastructure of infectious extracellular vesicles from cells infected by non-enveloped virus. [PDF]
Sci Rep, 2020 Enteroviruses support cell-to-cell viral transmission prior to their canonical lytic spread of virus. Poliovirus (PV), a prototype for human pathogenic positive-sense RNA enteroviruses, and picornaviruses in general, transport multiple virions en bloc ...
Yang JE +9 more
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