Results 71 to 80 of about 509,108 (267)

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Dual-Valence Copper Nanostructures with Cu⁺/Cu²⁺ Interfaces for High-Sensitivity Glucose Electrochemical Sensing

Nanomaterials
Copper-based materials, renowned for their redox versatility and conductivity, have extensive applications in electrochemical sensing. Herein, we construct stable Cu+/Cu2+ interfaces within dual-valence copper nanostructures to achieve enhanced ...
Zhipeng Yu, Pengxu Yan, Yilei Sheng, Chengwei Zhang, Zhun Qiao, Qikui Fan, Chuncai Kong, Zhimao Yang   +7 more
doaj   +1 more source

Numerical simulation of critical dissipative non-equilibrium quantum systems with an absorbing state

New Journal of Physics, 2019
The simulation of out-of-equilibrium dissipative quantum many body systems is a problem of fundamental interest to a number of fields in physics, ranging from condensed matter to cosmology.
Edward Gillman, Federico Carollo, Igor Lesanovsky   +2 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

CLASSICAL AND MULTISCALE NON-EQUILIBRIUM THERMODYNAMICS

Annals of the Academy of Romanian Scientists Series on Mathematics and Its Application
Classical and multiscale non-equilibrium thermodynamics have dif- ferent histories and different objectives. In this Note we explain the differences and review some topics in which the multiscale viewpoint of mesoscopic time evolution of macroscopic systems helped to advance the classical non-equilibrium thermodynamics.
Grmela, Miroslav, Pavelka, Michal
openaire   +3 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

NETS: A Non-Equilibrium Transport Sampler

CoRR
We propose an algorithm, termed the Non-Equilibrium Transport Sampler (NETS), to sample from unnormalized probability distributions. NETS can be viewed as a variant of annealed importance sampling (AIS) based on Jarzynski's equality, in which the stochastic differential equation used to perform the non-equilibrium sampling is augmented with an ...
Michael Samuel Albergo, Eric Vanden-Eijnden   +1 more
openaire   +3 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Noisy frequency estimation with noisy probes

New Journal of Physics, 2018
We consider frequency estimation in a noisy environment with noisy probes. This builds on previous studies, most of which assume that the initial probe state is pure, while the encoding process is noisy, or that the initial probe state is mixed, while ...
Agnieszka Górecka, Felix A Pollock, Pietro Liuzzo-Scorpo, Rosanna Nichols, Gerardo Adesso, Kavan Modi   +5 more
doaj   +1 more source