Results 41 to 50 of about 241,334 (299)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Theoretical and Numerical Analysis of the 3D-Distributed Thermodynamic Properties in Inclined Condenser Tubes

Chemical Engineering Transactions, 2022
Steam condensation in tubes exhibits obvious thermodynamic non-equilibrium characteristics, which leads to many problems, such as the decline of condenser efficiency and the freezing crack of condenser under low temperature.
Yuqian Wang, Chang Huang, Weiliang Wang
doaj   +1 more source

Kinetics of Strongly Non-equilibrium Bose-Einstein Condensation [PDF]

, 2007
We consider the ordering kinetics in a strongly non-equilibrium state of a (weakly) interacting Bose gas, characterized, on one hand, by large occupation numbers, and, on the other hand, by the absence of long-range order. Up to higher-order corrections in inverse occupation numbers, the evolution is described by non-linear Schrodinger equation with a ...
openaire   +2 more sources

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Current Status and Challenges in Data Collection for Aerospace Coatings Deposited by Plasma Spraying

Advanced Engineering Materials, EarlyView.
An innovative approach has been integrated into the GRENAT project to optimize plasma spraying and coating performance. Raw materials are accelerated and melted in the plasma generated by torches, creating coatings. Monitoring sensors collect process data which are combined with ex situ characterization data.
Lila Randriamananjara, Vincent Rat, Quentin Dubreuil, Patrice Valorge, Simon Goutier, Alain Denoirjean, Olivier Messe, Céline Ruelle, Jean‐Philippe Poli   +8 more
wiley   +1 more source

Turbulent meson condensation in quark deconfinement

Physics Letters B, 2015
In a QCD-like strongly coupled gauge theory at large Nc, using the AdS/CFT correspondence, we find that heavy quark deconfinement is accompanied by a coherent condensation of higher meson resonances.
Koji Hashimoto, Shunichiro Kinoshita, Keiju Murata, Takashi Oka   +3 more
doaj   +1 more source

The effect of the inlet steam superheat degree on the non-equilibrium condensation in steam turbine cascade

The Physics of Fluids
Due to the high steam velocity and low thermal parameters at the turbine's final stage, steam generates non-equilibrium condensation and forms a large number of small droplets during the process of pressure expansion.
Di Liang, Zining Ji, Yimin Li, Zhong-ning Zhou   +3 more
semanticscholar   +1 more source

Universality in Modelling Non-equilibrium Pattern Formation in Polariton Condensates [PDF]

, 2013
The key to understanding the universal behaviour of systems driven away from equilibrium lies in the common description obtained when particular microscopic models are reduced to order parameter equations. Universal order parameter equations written for complex matter fields are widely used to describe systems as different as Bose-Einstein condensates ...
Berloff, N. G, Keeling, J.
openaire   +2 more sources