Results 61 to 70 of about 15,914,423 (249)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Boundedness for iterated commutators of multilinear singular integrals of Dini's type on non-homogeneous metric measure spaces [PDF]

SCIENTIA SINICA Mathematica, 2017
Assume that $(\mathcal X, d, \mu)$ is a geometrically doubling metric space and the measure $\mu$ is an upper doubling measure. This paper focuses on studying the boundedness of iterated commutators, which is generated by multilinear singular integral operators with the function $\bm{b}$ in $\mathrm{RBMO}(\mu)$.
TAO XiangXing, ZHENG TaoTao
openaire   +1 more source

A metric approach to limit operators

, 2017
We extend the limit operator machinery of Rabinovich, Roch, and Silbermann from Z^N to (bounded geometry, strongly) discrete metric spaces. We do not assume the presence of any group structure or action on our metric spaces.
Spakula, Jan, Willett, Rufus
core   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Weak estimates for the maximal and Riesz potential operators on non-homogeneous central Morrey type spaces in L^1 over metric measure spaces

Annales Academiae Scientiarum Fennicae Mathematica, 2020
The authors provide weak \(M^{1,q,a}(X)\)-estimates for the maximal and Riesz potential operators. Under certain assumptions on the growth of the measures of balls in \(X\) (which hold in the Euclidean setting), it is shown in Theorems 3.3 and 4.6 that the maximal operator and the Riesz potential operator are bounded from \(M^{1,q,a}(X)\) to \(WM ...
Matsuoka, Katsuo, Mizuta, Yoshihiro, Shimomura, Tetsu   +2 more
openaire   +3 more sources

Gunk, Topology and Measure [PDF]

, 2004
I argue that it may well be the case that space and time do not consist of points, indeed that they have no smallest parts. I examine two different approaches to such pointless spaces (to 'gunky' spaces): a topological approach and a measure theoretic ...
Arntzenius, Frank
core  

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Boundedness of Commutators on Hardy Spaces over Metric Measure Spaces of Non-homogeneous Type

, 2015
34 pages, Submitted.
Lin, Haibo, Wu, Suqing, Yang, Dachun
openaire   +2 more sources