FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
The refinement of repeated exact time-to-event modeling: methodological refinements and implementation in NONMEM<sup>®</sup> -part I. [PDF]
Front PharmacolSeo J, Lim HS.
europepmc +1 more source
A comparison of non-parametric segmentation methods
, 2015 In image segmentation, level-set methods discriminating regions with Parzen estimates of their intensity distributions have proven useful in a broad variety of contexts. A number of area cost terms have been proposed to achieve this goal, such as log-likelihood, Bhattacharyya coefficient, Kullback-Leibler divergence and several others.
Sciolla, Bruno +4 more
openaire +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Stage-dependent timing of relative and absolute treatment benefit in transthyretin amyloid cardiomyopathy. [PDF]
Open HeartNabeshima Y +4 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
A non-parametric method to nowcast the Euro Area IPI [PDF]
Non-parametric methods have been empirically proved to be of great interest in the statistical literature in order to forecast stationary time series, but very few applications have been proposed in the econometrics literature.
Thomas Raffinot, Laurent Ferrara
core
Non-parametric differential methylation analysis characterizes histotype-specific promoter regions in epithelial ovarian cancer. [PDF]
EpigeneticsSwenson H +6 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source