Results 201 to 210 of about 11,612,374 (304)

Engineering a 660 nm‐Responsive Optogenetic Inducer of Pyroptosis for Precision Cancer Therapy

Advanced Science, EarlyView.
This study reports a bioorthogonal pyroptosis inducer, PyroRACS. By using a red‐light‐responsive Cre‐On genetic switch to control the expression of highly cytotoxic GSDMDNT, PyroRACS enables the artificial induction of pyroptosis with precise spatiotemporal control.
Mengkai Zhang, Zhichao Li, Yonghao Ma, Yangyang Sun, Wei Chen, Weiren Huang   +5 more
wiley   +1 more source

Long-read target enrichment sequencing for rAAV integration site analysis in engineered clones with targeted viral insertion. [PDF]

Mol Ther Nucleic Acids
Coratella E, Lemmens M, Oertli M, Gilbart Y, Davidsen K, Bontadelli L, Bohnert R, Rigal J, Bian J, Alpert T, Beibel M, Ruda V, Onishi-Seebacher M, Naumann U, Elhajouji A, Odermatt A, Hudry E, Libertini S.   +17 more
europepmc   +1 more source

Computation and Structure‐Guided Arginine Scanning Engineers a Hyperactive AP Endonuclease for Multiplex Viral RNA Sensing

Advanced Science, EarlyView.
Computational and structure‐guided arginine scanning rewires the DNA‐binding interface of APE1 to create APE1‐Evo, a hyperactive yet specific AP endonuclease. Integrated into the NAPTUNE‐V2.0 cascade, APE1‐Evo enables amplification‐free, multiplex viral RNA sensing for dengue virus and influenza A/B, highlighting a general strategy for engineering ...
Junlan Wang, Ting Wu, Feizuo Wang, Boyang Wang, Xin Yu Wong, Senfeng Zhang, Shengsheng Ma, Yao Liu, Fangyao Yao, Zhifeng Zeng, Siyuan Yang, Kunming Liu, Qi Wang, Yilong Wang, Yunxiang Zang, Xiaolong Wang, Xingxu Huang, Shao Li, Quanquan Ji, Tao Hu, Chunyi Hu   +20 more
wiley   +1 more source

Correction: Hwang et al. <i>IFN-β</i> Overexpressing Adipose-Derived Mesenchymal Stem Cells Mitigate Alcohol-Induced Liver Damage and Gut Permeability. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 8509. [PDF]

Int J Mol Sci
Hwang S, Eom YW, Kang SH, Baik SK, Kim MY.   +4 more
europepmc   +1 more source

ANGPTL4 Exacerbates Renal Injury in Diabetic Kidney Disease by Impairing Podocyte Lipophagy via Compromised Lysosomal Degradative Function

Advanced Science, EarlyView.
In diabetic kidney disease, elevated podocyte ANGPTL4 is linked to reduced TFEB nuclear localization and compromised lysosomal degradative function. These changes impair podocyte lipophagy and promote lipid‐droplet accumulation and podocyte injury, which may contribute to renal injury progression.
Xiaojing Liu, Shimin Jiang, Zhenkun Yang, Shunlai Shang, Jiayi Li, Guming Zou, Cheng Zhou, Wenge Li   +7 more
wiley   +1 more source

Systemic delivery of phagocytosis-shielded retroviral vectors enables in vivo HSC gene therapy for sickle cell disease. [PDF]

Cell Stem Cell
Klatt D, Mucci A, Liu B, Peschers M, Schiroli G, Schambach A, Manis J, Armant M, Scadden DT, Verhoeyen E, Pellin D, Williams DA, Brendel C.   +12 more
europepmc   +1 more source

Generation of a tumor- and tissue-specific episomal non-viral vector system. [PDF]

BMC Biotechnol, 2013
Haase R, Magnusson T, Su B, Kopp F, Wagner E, Lipps H, Baiker A, Ogris M.   +7 more
europepmc   +1 more source

A Blood‐Derived Factor Rescues ALS: Platelet Factor 4 Activates OPTN‐Dependent Autophagy to Clear SOD1 Aggregates Independently of PINK1

Advanced Science, EarlyView.
Systemic platelet factor 4 (PF4) is significantly depleted in amyotrophic lateral sclerosis (ALS). Peripheral PF4 replenishment restores central proteostasis by driving OPTN‐dependent, PINK1‐independent selective autophagy in motor neurons. This intervention effectively clears toxic SOD1 aggregates, blunts glial activation, and preserves neuromuscular ...
Qingjian Xie, Yanbo Zhu, Wenhua Jiang, Haobo Xie, Yaojia Li, Mengjie Hu, Kezheng Li, Huan Yu, Yixuan Pan, Chaoyi Jiang, Xueqin Song, Dongsheng Fan, Binbin Deng   +12 more
wiley   +1 more source

Temporal Rewiring of Innate Immunity by Vector-Borne Viruses for Host-Directed Antiviral Therapy. [PDF]

Int J Mol Sci
Kim E, Baur AS, Lee JH.
europepmc   +1 more source

Targeting the KCNC3‐Dependent Anterior Insular‐Lateral Orbitofrontal Cortex Glutamatergic Circuit Attenuates Post‐Traumatic Anxiety

Advanced Science, EarlyView.
Frontal lobe traumatic brain injury is associated with hyperactivity of an insular–orbitofrontal circuit in both patients and mice. By combination of integrating functional imaging, cell‐type–specific circuit manipulation, single‐cell transcriptomics, and whole‐cell recordings, this work identifies the downregulation of the potassium channel KCNC3 in ...
Meng‐Ge Li, Xiao‐Bo Qian, Hai‐Long Zhang, Di Li, Ling Ji, Xin‐Chun Xu, Jia‐Sheng Ding, Li‐Jun Yin, Li Zhang, Guang‐Yin Xu, Rong Gao   +10 more
wiley   +1 more source