Results 51 to 60 of about 20,553 (236)

Mesoporous‐Shell Monolayer Plasmonic Architecture Enables Quantitative and Decision‐Guided SERS

Advanced Science, EarlyView.
Mesoporous‐shell‐regulated molecular transport combined with monolayer hotspot determinism transforms surface‐enhanced Raman spectroscopy (SERS) from ultrasensitive detection into quantitative and decision‐guided molecular sensing. ABSTRACT Quantitative surface‐enhanced Raman spectroscopy (SERS) has long been impeded by stochastic hotspot formation ...
Guangyao Huang, Ying Wang, Yue Fang, Jingyuan Shao, Sensen Hao, Mancun Zhao, Yuan Yao, Junping Wang, Wei Zhou, Hongzhi Wang   +9 more
wiley   +1 more source

Synergistic Rescue of Nonsense Mutant Tumor Suppressor p53 by Combination Treatment with Aminoglycosides and Mdm2 Inhibitors

Frontiers in Oncology, 2018
The tumor suppressor gene TP53 is inactivated by mutation in a large fraction of human tumors. Around 10% of TP53 mutations are nonsense mutations that lead to premature termination of translation and expression of truncated unstable and non-functional ...
Meiqiongzi Zhang, Angelos Heldin, Mireia Palomar-Siles, Susanne Öhlin, Vladimir J. N. Bykov, Klas G. Wiman   +5 more
doaj   +1 more source

Robust Cell-Free Expression of Sub-Pathological and Pathological Huntingtin Exon-1 for NMR Studies. General Approaches for the Isotopic Labeling of Low-Complexity Proteins

Biomolecules, 2020
The high-resolution structural study of huntingtin exon-1 (HttEx1) has long been hampered by its intrinsic properties. In addition to being prone to aggregate, HttEx1 contains low-complexity regions (LCRs) and is intrinsically disordered, ruling out ...
Anna Morató, Carlos A. Elena-Real, Matija Popovic, Aurélie Fournet, Karen Zhang, Frédéric Allemand, Nathalie Sibille, Annika Urbanek, Pau Bernadó   +8 more
doaj   +1 more source

Engineered tRNAs suppress nonsense mutations in cells and in vivo

Nature, 2023
Abstract Nonsense mutations are the underlying cause of approximately 11% of all inherited genetic diseases 1 . Nonsense mutations convert a sense codon that is decoded by tRNA into a premature termination codon (PTC), resulting in an abrupt termination of translation.
Suki Albers, Elizabeth C. Allen, Nikhil Bharti, Marcos Davyt, Disha Joshi, Carlos G. Perez-Garcia, Leonardo Santos, Rajesh Mukthavaram, Miguel Angel Delgado-Toscano, Brandon Molina, Kristen Kuakini, Maher Alayyoubi, Kyoung-Joo Jenny Park, Grishma Acharya, Jose A. Gonzalez, Amit Sagi, Susan E. Birket, Guillermo J. Tearney, Steven M. Rowe, Candela Manfredi, Jeong S. Hong, Kiyoshi Tachikawa, Priya Karmali, Daiki Matsuda, Eric J. Sorscher, Pad Chivukula, Zoya Ignatova   +26 more
openaire   +2 more sources

The influence of the reading context upon the suppression of nonsense codons

Molecular and General Genetics MGG, 1969
We have examined the response of phage T4 nonsense mutations located at various sites within the same cistron to different suppression agents. A wide range of suppression efficiency is found for both ochre (UAA) and amber (UAG) mutations under conditions where suppression provides a measurement of the amount of chain propagation past the mutated site ...
M M, Fluck, W, Salser, R H, Epstein
openaire   +4 more sources

Translation velocity determines the efficacy of engineered suppressor tRNAs on pathogenic nonsense mutations

Nature Communications
Nonsense mutations – the underlying cause of approximately 11% of all genetic diseases – prematurely terminate protein synthesis by mutating a sense codon to a premature stop or termination codon (PTC).
Nikhil Bharti, Leonardo Santos, Marcos Davyt, Stine Behrmann, Marie Eichholtz, Alejandro Jimenez-Sanchez, Jeong S. Hong, Andras Rab, Eric J. Sorscher, Suki Albers, Zoya Ignatova   +10 more
doaj   +1 more source

Electrically Tunable Graphene‐Metal Hybrid Metasurfaces for High‐Efficiency, Broadband Mid‐Infrared Transmission Modulation

Advanced Science, EarlyView.
A graphene‐metal hybrid transmissive metasurface enables electrically tunable mid‐infrared transmission modulation through ionic gel top gating. By combining graphene with a metallic slit metasurface, high modulation efficiency and broadband operation are achieved simultaneously, demonstrating efficient electrical control of mid‐infrared transmission ...
Heonhak Ha, Jinseok Kong, Junhyung Kim, Jiwon Kang, Min Seok Jang   +4 more
wiley   +1 more source

Suppression of Nonsense Mutations in Rett Syndrome by Aminoglycoside Antibiotics [PDF]

Pediatric Research, 2009
Rett Syndrome (RTT) is caused in more than 60% of cases by nonsense mutations in the MECP2 gene. So far, no curative therapy for RTT has become available. In other genetic disorders, it has been shown that aminoglycosides can cause a read-through of nonsense mutations with an efficiency of up to 20%.
Brendel, Cornelia, Klahold, Edith, Gärtner, Jutta, Huppke, Peter   +3 more
openaire   +3 more sources

Ab Initio Thermoelectric Transport Calculations of Alloyed Half‐Heuslers

Advanced Science, EarlyView.
The effect of alloying on the electronic transport of half‐Heusler thermoelectric materials is explored using a fully ab initio approach. A novel method is developed that combines calculations of alloyed supercells with Boltzmann transport, considering the full energy and momentum dependent scattering processes from first principles extracted ...
Ankit Kumar, Bhawna Sahni, Neophytos Neophytou   +2 more
wiley   +1 more source

Site‐Specific Protein Bioconjugation Through Cellular Incorporation of Noncanonical Amino Acids

Angewandte Chemie, EarlyView.
Genetic code expansion (GCE) enables site‐specific installation of noncanonical amino acids containing bioorthogonal conjugation handles, allowing precise, homogeneous protein modification. This review examines the principles of orthogonal translation, surveys the chemistries available for chemoselective labeling, and highlights emerging multi‐site ...
Rahul Sarkar, Manas Joshi, Abhishek Chatterjee   +2 more
wiley   +2 more sources