Optimized RT-qPCR and a novel normalization method for validating circulating miRNA biomarkers in ageing-related diseases. [PDF]
Sci Rep, 2023 Circulating miRNAs have potential as minimally invasive biomarkers for diagnosing various diseases, including ageing-related disorders such as Alzheimer’s disease (AD).
Want A +8 more
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Selecting Normalizers for MicroRNA RT-qPCR Expression Analysis in Murine Preimplantation Embryos and the Associated Conditioned Culture Media. [PDF]
J Dev Biol, 2023 Normalizing RT-qPCR miRNA datasets that encompass numerous preimplantation embryo stages requires the identification of miRNAs that may be used as stable reference genes.
Hawke DC, Watson AJ, Betts DH.
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Identification of extremely GC-rich micro RNAs for RT-qPCR data normalization in human plasma. [PDF]
Front Genet, 2022 We aimed at extending the repertoire of high-quality miRNA normalizers for reverse transcription-quantitative PCR (RT-qPCR) of human plasma with special emphasis on the extremely guanine-cytosine-rich portion of the miRNome. For high-throughput selection
Baumann V +5 more
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A Note on the First Integrals of Vector Fields with Integrating Factors and Normalizers [PDF]
Symmetry, Integrability and Geometry: Methods and Applications, 2012 We prove a sufficient condition for the existence of explicit first integrals for vector fields which admit an integrating factor. This theorem recovers and extends previous results in the literature on the integrability of vector fields which are volume
Jaume Llibre, Daniel Peralta-Salas
doaj +6 more sources
MiR-151a: a robust endogenous control for normalizing small extracellular vesicle cargo in human cancer. [PDF]
Biomark Res, 2023 Small extracellular vesicles (sEVs) in the blood of cancer patients contain higher amounts of tumor markers than those identified as free-circulating.
Burdiel M +17 more
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Normalizers and self-normalizing subgroups II
Open Mathematics, 2011 Abstract Let $\mathbb{K}$ be a field, G a reductive algebraic $\mathbb{K}$-group, and G 1 ≤ G a reductive subgroup. For G 1 ≤ G, the corresponding groups of $\mathbb{K}$-points, we study the normalizer N = N G(G 1).
Širola Boris
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Wastewater Surveillance for Group A <i>Streptococcus pyogenes</i> in a Small City. [PDF]
PathogensStreptococcus pyogenes is a bacterial pathogen known to be the causative agent in many different illnesses, with Group A Streptococcus (GAS) pharyngitis (strep throat), being one of the more prevalent.
Birch ON, Garza FM, Greaves JC.
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Identification of SnRNA U6 as an endogenous reference gene for normalization of MiRNA expression data in COVID-19 patients. [PDF]
Sci RepSARS-CoV-2, the virus responsible for COVID-19, exhibits structural differences compared to other coronaviruses that impact transmission dynamics and host response.
de Santana Silva ÍTS +10 more
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Data normalization of plasma miRNA profiling from patients with COVID-19. [PDF]
Sci RepWhen using the reverse-transcription quantitative polymerase chain reaction (RT-qPCR) technique for quantitative assessment of microRNA (miRNA) expression, normalizing data using a stable endogenous gene is essential; however, no universally adequate ...
Siguemoto JT +12 more
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cdev: a ground-truth based measure to evaluate RNA-seq normalization performance [PDF]
PeerJ, 2021 Normalization of RNA-seq data has been an active area of research since the problem was first recognized a decade ago. Despite the active development of new normalizers, their performance measures have been given little attention. To evaluate normalizers,
Diem-Trang Tran, Matthew Might
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