Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
A Divide-and-Conquer Embedded Correlated Wavefunction Transfer Learning Approach for Chemically Accurate Condensed-Phase Interatomic Potentials: Liquid Water as a Test Case. [PDF]
J Phys Chem LettBian X, Carter EA.
europepmc +1 more source
, 2014 Cross sections for neutron capture in the range of unresolved resonances are predicted simultaneously to level distances at the neutron threshold for more than 100 spin-0 target nuclei with A >70.
Grosse, Eckart +2 more
core
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Computational Screening of Infrared Resonant Bands in Cholesterol Monohydrate for Selective Laser Lithotripsy of Cholesterol Gallstones. [PDF]
ACS OmegaXia Y +8 more
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
FEBS Open Bio, EarlyView.GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
Cycloastragenol Attenuates Cisplatin-Induced Ovarian Toxicity in Rats: Associations With TERT Expression, Apoptotic Balance, and FOXO3a-mTOR-Related Signaling. [PDF]
J Biochem Mol ToxicolErol GNA, Yar AS, Kaplanoğlu GT.
europepmc +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
FEBS Open Bio, EarlyView.Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
A deep neural network-enhanced multi-scale coupled model of patient-specific acoustic wave propagation for mechanotransduction stimulation of healthy and osteoarthritic knee articular cartilage. [PDF]
Biomech Model MechanobiolFernandes NATC +3 more
europepmc +1 more source