Extremely rapid and reversible optogenetic perturbation of nuclear proteins in living embryos.
Kögler AC +5 more
europepmc +1 more source
Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation. [PDF]
Cattoglio C +4 more
europepmc +1 more source
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama +7 more
wiley +1 more source
Biology of PEST-containing nuclear proteins as potential targets in ovarian cancer (Review). [PDF]
Liu H +10 more
europepmc +1 more source
Characterization of a Nuclear Protein Conferring Brefeldin A Resistance in Schizosaccharomyces pombe [PDF]
Thomas G. Turi +3 more
openalex +1 more source
A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms
We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley +1 more source
Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cells. [PDF]
Tseng PL +4 more
europepmc +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Binding of 14-3-3 proteins and nuclear export control the intracellular localization of the mitotic inducer Cdc25 [PDF]
Akiko Kumagai, William G. Dunphy
openalex +1 more source

