Results 111 to 120 of about 6,528,274 (245)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

ENDF/B-VIII.0: The 8 th Major Release of the Nuclear Reaction Data Library with CIELO-project Cross Sections, New Standards and Thermal Scattering Data

, 2018
D. Brown, M. Chadwick, R. Capote, A. Kahler, A. Trkov, M. Herman, A. Sonzogni, Y. Danon, A. Carlson, M. Dunn, D. L. Smith, G. Hale, G. Arbanas, R. Arcilla, C. Bates, B. Beck, B. Becker, F. Brown, R. Casperson, J. Conlin, D. Cullen, M. Descalle, R. Firestone, T. Gaines, K. Guber, A. Hawari, J. Holmes, T. Johnson, T. Kawano, B. Kiedrowski, A. Koning, S. Kopecky, L. Leal, J. Lestone, C. Lubitz, J. Damián, C. Mattoon, E. McCutchan, S. Mughabghab, P. Navrátil, D. Neudecker, G. Nobre, G. Noguere, Mark Wayne Paris, M. Pigni, A. Plompen, B. Pritychenko, V. Pronyaev, D. Roubtsov, D. Rochman, P. Romano, P. Schillebeeckx, S. Simakov, M. Sin, I. Sirakov, B. Sleaford, V. Sobes, E. Soukhovitskiĩ, I. Stetcu, P. Talou, I. Thompson, S. V. Marck, L. Welser-Sherrill, D. Wiarda, M. White, J. Wormald, R. Q. Wright, M. Zerkle, G. Zerovnik, Y. Zhu   +69 more
semanticscholar   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source

TMC4 localizes to multiple taste cell types in the mouse taste papillae

FEBS Open Bio, EarlyView.
Transmembrane channel‐like 4 (TMC4), a voltage‐dependent chloride channel, plays a critical role in amiloride‐insensitive salty taste transduction. TMC4 is broadly expressed in all mature taste cell types, suggesting a possible involvement of multiple cell types in this pathway.
Momo Murata, Yoshikazu Saito, Yoichi Kasahara, Wataru Fujii, Takumi Misaka, Tomiko Asakura, Masataka Narukawa   +6 more
wiley   +1 more source

Formation and decay of resonance state in 9Be and 9B nuclei. Microscopic three-cluster model investigations

Physical Sciences and Technology, 2017
We study nature of the low-lying resonance states in mirror nuclei 9Be and 9B. Investigations are performed within a three-cluster model. The model make uses of the hyperspherical harmonics, which provide convenient description of three-cluster continuum.
V. S. Vasilevsky, K. Kato, N. Zh. Takibayev   +2 more
doaj  

Research progress on the metabolic transformation of Shenling Baizhu San under the influence of the gut microbiota

Advanced Chinese Medicine, EarlyView.
Abstract The gut microbiota plays a pivotal role in maintaining host metabolic homeostasis and overall physiological functions. Shenling Baizhu San (SLBZS), a time‐honored classical prescription for tonifying Qi, strengthening the spleen, removing dampness, and arresting diarrhea, has shown broad clinical efficacy in digestive disorders.
Jinlan Di, Xiaochun Zhang
wiley   +1 more source

Towards a More Complete and Accurate Experimental Nuclear Reaction Data Library (EXFOR): International Collaboration Between Nuclear Reaction Data Centres (NRDC) [PDF]

, 2014
N. Otuka, E. Dupont, V. Semkova, B. Pritychenko, A. Blokhin, M. Aikawa, S. Babykina, M. Bossant, G. Chen, S. Dunaeva, R. Forrest, T. Fukahori, N. Furutachi, S. Ganesan, Z. Ge, O. Gritzay, M. Herman, S. Hlavavc, K. Katō, B. Lalremruata, Y. O. Lee, A. Makinaga, K. Matsumoto, M. Mikhaylyukova, G. Pikulina, V. Pronyaev, A. Saxena, O. Schwerer, S. Simakov, N. Soppera, R. Suzuki, S. Takács, X. Tao, S. Taova, F.Tarkanyi, V. Varlamov, J. Wang, S. C. Yang, V. Zerkin, Y. Zhuang   +39 more
semanticscholar   +1 more source

Progress of research on delaying bone destruction of rheumatoid arthritis by combining traditional Chinese and Western medicine

Advanced Chinese Medicine, EarlyView.
Abstract Rheumatoid arthritis (RA) is a common autoimmune disease with bone destruction as the core pathology, which belongs to the category of “impediment disease” in traditional Chinese medicine (TCM), and its etiology and pathogenesis are still not completely clear.
Dier Jin, Yuanyuan Xu, Wenwen Qi, Tianyi Lan, Ting Liu, Rihong Liu, Zihan Wang, Yuan Xu   +7 more
wiley   +1 more source