Results 81 to 90 of about 2,571,740 (262)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Drug-nucleic acid interactions /
, 2001 This volume consolidates the key methods for studying ligand-nucleic acid interactions into a convenient source. Techniques that are examined range from biophysical and chemical approaches to methods rooted in molecular and cell biology.Includes ...
Chaires, Jonathan B.,editor. +1 more
core
Plant pathogen diagnostics: immunological and nucleic acid-based approaches
, 2004 Accurate disease diagnosis and precise identification of any pathogens involved is an essential prerequisite for understanding plant diseases and controlling them effectively.
Fraaije, B. A. +3 more
core +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Re-Evaluating the Spherical-Nucleic-Acid Technology [PDF]
, 2018 We re-evaluate the evidence presented for the detection of mRNA in cells and tissues with Spherical Nucleic Acids.
Víctor F., Puntes +6 more
core +1 more source
FEBS Open Bio, EarlyView.Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar +11 more
wiley +1 more source
Fast and sensitive determination of camel's and goat's meat and milk using species-specific genetic markers [PDF]
Biotechnology in Animal Husbandry, 2015 For the fast and sensitive determination of camel's and goat's meat and milk, species-specific regions (SSR) of follicle stimulating hormone receptor (FSHR) gene in both camel and goat were amplified using PCR technique.
Abdel-Rahman S.M. +2 more
doaj
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
FEBS Open Bio, EarlyView.Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
Programmed nucleic acid assembly for nanomedicines
, 2022 S.116-131Nanometer-scale therapeutic agents provide the possibility for a degree of cellular interactions not available with conventional medicines. The gold standard for innovations in such nanomedicines is what early 20th century German physician Paul ...
Smith, D.
core +1 more source