Results 211 to 220 of about 186,936 (344)

MARPLE: A Proximity‐Triggered CRISPR‐Cas13 Platform for Ultrasensitive Antibody Detection

Advanced Science, EarlyView.
MARPLE is a modular CRISPR‐Cas13 immunoassay that uses antibody‐induced proximity to trigger a toehold‐exchange reaction, releasing an RNA activator for Cas13. This one‐pot, isothermal architecture enables ultrasensitive (femtomolar) and highly specific detection of diverse antibodies, including therapeutic, pathogen‐specific, and cancer‐associated ...
Elena Spezzani, Luca Capelli, Denise Di Lena, Alejandro Chamorro‐Garcia, Rudy Ippodrino, Alessandro Porchetta, Alessandro Bertucci   +6 more
wiley   +1 more source

Advances in the application of CRISPR technology in pathogen detection: amplification-based and amplification-free strategies. [PDF]

Front Cell Infect Microbiol
Zhou X, Ye C, Xie M, Wei Y, Zhao Y, Liu X, Ma J, Qing J, Chen Z.   +8 more
europepmc   +1 more source

Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory [PDF]

, 1997
M. Ieven, Herman Goossens
openalex   +1 more source

Dynamic Decoration of DNA Scaffolds for High‐Resolution Cancer Cell Subtyping

Advanced Science, EarlyView.
This study presents a dual‐mode imaging platform that utilizes self‐assembly and disassembly of DNA scaffolds for high‐resolution cancer cell detection. The structural rigidity of DNA scaffolds effectively prevents cellular internalization for over 300 min.
Xiaolin Hu, Jie Xie, Xinlin Guo, Liangting Wang, Zhengheng Yu, Xiaopei Qiu, Heng Li, Kang Wang, Xiaoxing Wang, Mingxuan Song, Junsong Guo, Wei Gu, Sergio Bernardini, Chaoyong Yang, Hong Zhang, Yang Luo   +15 more
wiley   +1 more source

Collaborative study to evaluate a candidate World Health Organization international standard for chikungunya virus for nucleic acid amplification technique (NAT)-based assays

, 2017
Julia Kreβ, K-M.O. Hanschmann, M. Chudy
openalex   +1 more source

Electrochemical aptamer-based assays coupled to isothermal nucleic acid amplification techniques: New tools for cancer diagnosis

, 2018
Ramón Lorenzo-Gómez, Rebeca Miranda‐Castro, Noemí de‐los‐Santos‐Álvarez, María Jesús Lobo‐Castañón   +3 more
openalex   +2 more sources

Engineered Small Extracellular Vesicles Targeting Tumor‐Associated Endothelial Cells to Effectively Remodel the Glioma Microenvironment

Advanced Science, EarlyView.
This study developed a novel drug delivery platform that overcomes the blood‐brain barrier in glioblastoma. By fusing IGFBP7 with small extracellular vesicles, the platform specifically targets tumor vasculature. It effectively delivers temozolomide, suppressing tumor growth at low doses.
Lingling Liu, Feiyang Xu, Zhiming Zheng, Xiaodan Yang, Fang Yang, Pei Liu, Yuankun Chen, Yunshu Yang, Junli Zhao, Peiyan Yang, Xiaojing Zheng, Xiaohong Sun, Ping Mao, Qinwen Mao, Hao Guan, Haibin Xia, Weifeng Zhang, Dan Xiao   +17 more
wiley   +1 more source

A Portable Dual-Mode Microfluidic Device Integrating RT-qPCR and RT-LAMP for Rapid Nucleic Acid Detection in Point-of-Care Testing. [PDF]

Biosensors (Basel)
Zhang B, Li X, Huang M, Jiang M, Du L, Yin P, Fang X, Jiang X, Qi F, Lin Y, Ma F.   +10 more
europepmc   +1 more source

An international collaborative study to establish a World Health Organization international standard for hepatitis B virus DNA nucleic acid amplification techniques

, 2001
J. Saldanha, Wolfram H. Gerlich, Nico Lelie, P J Dawson, K.‐H. Heermann, Alan Heath, The WHO Collaborative Study Group   +6 more
openalex   +2 more sources

Biomimetic Membrane Interface Technologies for Detection and Isolation of CTCs and EVs: Advances and Opportunities in Liquid Biopsy

Advanced Science, EarlyView.
Biomimetic membrane interface engineering constructs functionalized detection platforms by integrating natural cell membranes, synthetic lipids, or hybrid membranes. This strategy effectively reduces background interference and enables efficient target capture and analysis, showing broad applications in circulating tumor cell separation, extracellular ...
Duo Liu, Jie Yu, Jingxue Li, Yixue Chen, Mengle Peng, Yifan Xu, Yongjun Wu, Lihua Ding, Sitian He   +8 more
wiley   +1 more source