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Amplification-Based Nucleic Acid Scanning Techniques to Assess Genetic Polymorphism inCandida
Critical Reviews in Microbiology, 2003Opportunistic pathogen Candida causes common fungal infections that manifest both superficially and systemically, especially in compromised patients. Although C. albicans is by far the main etiological agent of candidosis, the frequency of isolation of other non-albicans species such as C. glabrata and C.
Dassanayake, RS, Samaranayake, LP
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An Introduction to In Vitro Nucleic Acid Amplification Techniques
2012A molecular microbiology detection procedure consists of three basic components: (1) nucleic acid extraction, (2) amplification, and (3) detection/identification. Over the past two decades, the development and optimization of a series of in vitro nucleic acid amplification (NAA) technologies has opened new avenues in diagnostic microbiology for the ...
Haijing Li, Yi-Wei Tang
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Research and Application Progress of Digital Nucleic Acid Amplification Detection Techniques
Chinese Journal of Analytical Chemistry, 2016Abstract Recently, micro/nano-scaled fluidic control technologies have been developed to be the alternative to traditional analysis approaches due to the capability of realizing miniaturized multiphase and multistep reactions. The perfect combination of these techniques and nucleic acid amplification methods effectively promotes the establishment and
Xiong DING, Ying MU
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Concordance between nucleic acid amplification technique and culture for the diagnosis of gonorrhoea
International Journal of STD & AIDS, 2009The objective of the study was to evaluate the concordance between nucleic acid amplification technique (NAAT) and culture for the diagnosis of Neisseria gonorrhoeae among attendees at a genitourinary medicine clinic in East London. All patients testing positive for N.
S, Creighton, B, Revell, A, Barrow
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Analytical Biochemistry, 2020
The advancement in developing sensitive, rapid, and specific sensing tools is crucial in diagnostics and biotechnological applications. Although various isothermal amplification approaches exist for the detection and identification of nucleic acids, post-amplicon analysis is still based on traditional methods such as gel electrophoresis, colorimetry ...
Omar, Mukama +10 more
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The advancement in developing sensitive, rapid, and specific sensing tools is crucial in diagnostics and biotechnological applications. Although various isothermal amplification approaches exist for the detection and identification of nucleic acids, post-amplicon analysis is still based on traditional methods such as gel electrophoresis, colorimetry ...
Omar, Mukama +10 more
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Mycoses, 2020
AbstractBackgroundFungal infections have increased during the last years due to the AIDS epidemic and immunosuppressive therapies. The available diagnostic methods, such as culture, histopathology and serology, have several drawbacks regarding sensitivity, specificity and time‐consuming, while molecular methods are still expensive and dependent on many
Matheus Silva Zatti +2 more
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AbstractBackgroundFungal infections have increased during the last years due to the AIDS epidemic and immunosuppressive therapies. The available diagnostic methods, such as culture, histopathology and serology, have several drawbacks regarding sensitivity, specificity and time‐consuming, while molecular methods are still expensive and dependent on many
Matheus Silva Zatti +2 more
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Folia Microbiologica, 2007
An improved nucleic acid amplification test (NAAT) to detect Chlamydia trachomatis infections, based on PCR amplification within its cryptic plasmid (CT1/CT2 Test) was developed. DNA was extracted from urogenital swabs and a 594-bp long DNA fragment from the cryptic plasmid (pCT) was amplified.
Biroš, E. +9 more
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An improved nucleic acid amplification test (NAAT) to detect Chlamydia trachomatis infections, based on PCR amplification within its cryptic plasmid (CT1/CT2 Test) was developed. DNA was extracted from urogenital swabs and a 594-bp long DNA fragment from the cryptic plasmid (pCT) was amplified.
Biroš, E. +9 more
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Isothermal Amplification Techniques for the Detection of Nucleic Acids and Proteins
2020The early detection of disease is beneficial for improved patient prognoses. One major challenge in early disease detection is the small, undetectable amounts of biomarkers. Detection strategies that confer amplification of the biomarker itself or of the detection signal are therefore desirable. Furthermore, emerging strategies conferring amplification
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Broad-Spectrum Molecular Detection of Fungal Nucleic Acids by PCR-Based Amplification Techniques
2016Over the past decade, the incidence of life-threatening invasive fungal infections has dramatically increased. Infections caused by hitherto rare and emerging fungal pathogens are associated with significant morbidity and mortality among immunocompromised patients.
Stefan, Czurda, Thomas, Lion
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Nucleic acid amplification and related techniques in microbiological diagnostics and epidemiology.
Cellular and molecular biology (Noisy-le-Grand, France), 1995The use of nucleic acid amplification techniques within the medical microbiology laboratory is becoming more and more accepted. Polymerase chain reaction (PCR) tests or nucleic acid sequence based amplification (NASBA) assays are already available in the form of commercial kits.
A, Van Belkum, H G, Niesters
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