Results 161 to 170 of about 183,908 (336)

Lateral Flow Biosensor for On-Site Multiplex Detection of Viruses Based on One-Step Reverse Transcription and Strand Displacement Amplification

Biosensors
Respiratory pathogens pose a huge threat to public health, especially the highly mutant RNA viruses. Therefore, reliable, on-site, rapid diagnosis of such pathogens is an urgent need.
Xuewen Lu, Kangning Ding, Zhiyuan Fang, Yilei Liu, Tianxing Ji, Jian Sun, Zhenling Zeng, Limin He   +7 more
doaj   +1 more source

Design and Characterization of DX‐Tile DNA Nanostar‐Based Hydrogels

Advanced Science, EarlyView.
In this study, the authors demonstrated that DNA DX‐tile‐based multi‐arm motifs can be used to assemble pure DNA hydrogels, offering greater design flexibility and enhanced control over their mechanical properties and functionalization capabilities compared to single duplex‐based DNA hydrogels.
Dylan V. Scarton, Alessandra B. Coogan, Peter M. Touma, Eray O. Tulun, Katie A. Harrison, Jack Buchen, Richard C. Steiner, Christopher R. Fellin, Hunter G. Mason, Chih‐Hsiang Hu, Sally Farag, Xiaoning Yuan, Shailly Jariwala, Remi Veneziano   +13 more
wiley   +1 more source

NPM1 Mediates mRNA Sorting into Extracellular Vesicles via Specific RNA Motif Binding and Phase Separation

Advanced Science, EarlyView.
NPM1 selectively sorts specific mRNAs into extracellular vesicles (EVs) by recognizing RNA motifs and forming phase‐separated condensates. These mRNA cargos—including EGFR—are then loaded via multivesicular bodies. This active sorting pathway, validated in EVs derived from both lung cancer cells and patient serum, reveals a specific mechanism for ...
Kaixiang Zhang, Gaoge Sun, Xiangzheng Liu, Shuai Zuo, Pengyuan Wang, Boxiao Zhan, Zhi John Lu, Hang Yin, Ying Zhang   +8 more
wiley   +1 more source

A gamepad-like nucleic acid testing device for rapid detection of SARS-CoV-2 via visible nested recombinase polymerase amplification

Communications Engineering
Nucleic acid tests are essential for the accurate diagnosis and control of infectious diseases. However, current assays are not easily scalable for a large population, due to the requirement of laboratory settings or special equipment. Here, we developed
Ce Liu, Baobao Lin, Guohua Yuan, Zhi Geng, Zhe Zhao, Jiacheng Wang, Jingyu Shao, Zhenqi Wang, Yang Xu, Xujuan Yang, Chang Liu, Yingmei Feng, Xin Fan, Jing Wang, Lili Ren, Yan Xiao, Conghui Wang, Guang Shen, Yi Yang, Chao Zhao, Yinqing Li, Peng Liu, Jingwei Bai   +22 more
doaj   +1 more source

An Insulin‐Exosome‐TNFAIP8 Axis Drives Stromal Fibrosis and Therapeutic Resistance in Pancreatic Cancer

Advanced Science, EarlyView.
In pancreatic ductal adenocarcinoma (PDAC), insulin is associated with activation of PI3K/AKT–RAB3A signaling and enhanced secretion of TNFAIP8‐enriched exosomes from tumor cells. Uptake of these exosomes by fibroblasts is linked to TRIM21‐dependent STAT1 degradation and the emergence of myofibroblastic CAF–associated features, accompanied by increased
Zhenyu Li, Li Chen, Tao Wang, Haiyang Jiang, Huijuan Wang, Mengyu Li, Guanpeng Xie, Chunhua Xi, Han Yan, Chunhui Lu, Chenchen Li, Hanyu Zhu, Feihu Sun, Lingdi Yin, Jun Yu, Yi Miao   +15 more
wiley   +1 more source

Proportion of Microbiologically Confirmed Extrapulmonary Tuberculosis among Pathologically diagnosed Extrapulmonary Tuberculosis

Journal of Advanced Lung Health
Context: Current National Tuberculosis Elimination Program (NTEP) guidelines recommends culture/CBNAAT/molecular test/histopathology examination and drug sensitivity testing for all patients with presumptive Extrapulmonary TB (EPTB).
Lesitha Sivaraman, Aiswarya Mukundan, Anitha Thattamparambil Ravindranathan, M Surekha Vijayan, Vishnu Kuruveettil, Sanjeev Nair, Thomas George   +6 more
doaj   +1 more source

Paper microfluidics with deep learning for portable intelligent nucleic acid amplification tests. [PDF]

Talanta, 2023
Sun H, Xie W, Huang Y, Mo J, Dong H, Chen X, Zhang Z, Shang J.   +7 more
europepmc   +1 more source

Evaluation of the prevalence of sexually transmitted bacterial pathogens in *** by nucleic acid amplification tests and investigation of the relationship between these pathogens and cervicitis

, 2019
Onur Güralp, Ayşegül Bostancı, Esra Ozerkman Basaran, Meike Schild-Suhren, Barış Kaya   +4 more
openalex   +1 more source

An Artificial Antibody‐Based Toolbox Accelerates Validation of Hidden Microproteins Encoded by the Dark Genome

Advanced Science, EarlyView.
Microproteins are hidden treasures encoded by the “dark proteome” but remain largely underexplored due to the lack of highly efficient tools. We developed a molecularly imprinted polymers (MIPs)‐based toolbox (CLAIMID) to achieve accelerated and ultrasensitive microproteins validation at multiple biological scales (single living cells, cell populations,
Hui He, Anqi Zhang, Zhanchen Guo, Qi Li, Song Gao, Wenyan Guan, Ying Li, Jingran Chen, Sijia Sun, Jian He, Zhen Liu   +10 more
wiley   +1 more source

Optimal Sampling Strategy for the Detection of C.Trachomatis (CT) and N.Gonorrhoeae (NG) in Adolescent Females Using Nucleic Acid Amplification Testing [PDF]

, 2013
Élise Dubuc, Kaede V. Ota, Trisha Tulloch, Yolanda A. Kirkham, Erin Barlow, Tania Dumont, Carolyn Hoyle, Catherine Maser, Yvonne Yau, Debra K. Katzman, Susan E. Richardson, Lisa Allen   +11 more
openalex   +1 more source