Development of an Automated, Non-Enzymatic Nucleic Acid Amplification Test
Micromachines, 2021 Among nucleic acid diagnostic strategies, non-enzymatic tests are the most promising for application at the point of care in low-resource settings. They remain relatively under-utilized, however, due to inadequate sensitivity. Inspired by a recent demonstration of a highly-sensitive dumbbell DNA amplification strategy, we developed an automated, self ...
Zackary A. Zimmers +4 more
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Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes
BioTechniques, 2015 Nucleic acid amplification is the basis for many molecular diagnostic assays. In these cases, the amplification product must be detected and analyzed, typically requiring extended workflow time, sophisticated equipment, or both.
Nathan A. Tanner +2 more
doaj +1 more source
Forty years of Tuberculous meningitis: The new face of an old enemy
International Journal of Infectious Diseases, 2020 Background: Tuberculous meningitis (TBM) occurs in 1–5% of cases of tuberculosis. Without early treatment, mortality and permanent disability rates are high.
A. García-Caballero +7 more
doaj +1 more source
A Simple, Low-Cost Platform for Real-Time Isothermal Nucleic Acid Amplification
Sensors, 2015 Advances in microfluidics and the introduction of isothermal nucleic acid amplification assays have resulted in a range of solutions for nucleic acid amplification tests suited for point of care and field use.
Pascal Craw +6 more
doaj +1 more source
Loop-mediated isothermal amplification (LAMP) for the rapid detection of Mycoplasma genitalium [PDF]
Mycoplasma genitalium is a sexually transmissible, pathogenic bacterium and a significant cause of nongonococcal urethritis in both men and women. Due to the difficulty of the culture of M. genitalium from clinical samples, the laboratory diagnosis of M.
Burke, P +6 more
core +1 more source
Nucleic Acid Amplification Tests for the Diagnosis of Pneumonia [PDF]
Clinical Infectious Diseases, 2003 Molecular diagnostic techniques, such as polymerase chain reaction (PCR), are promising tools for the rapid etiological diagnosis of pneumonia. PCR offers potential advantages over conventional tests for the detection of Mycoplasma pneumoniae, Legionella species, and Chlamydia pneumoniae.
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Coupling Sensitive Nucleic Acid Amplification with Commercial Pregnancy Test Strips [PDF]
Angewandte Chemie International Edition, 2016 AbstractThe detection of nucleic acid biomarkers for point‐of‐care (POC) diagnostics is currently limited by technical complexity, cost, and time constraints. To overcome these shortcomings, we have combined loop‐mediated isothermal amplification (LAMP), programmable toehold‐mediated strand‐exchange signal transduction, and standard pregnancy test ...
Yan, Du +5 more
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Nucleic Acid Amplification Tests for Diagnosis of Bordetella Infections [PDF]
Journal of Clinical Microbiology, 2005 The global epidemiology of pertussis has recently been reviewed (12, 15). Bordetella pertussis continues to circulate even in populations where high vaccination coverage of infants and children is achieved (15, 23), because the protection after natural infection wanes after 10 to 15 years and protection after vaccination lasts for 6 to 10 years (15). A
Riffelmann, M. +3 more
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Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeriamonocytogenes in food [PDF]
, 2009 We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with
Amerongen, A., van +3 more
core +2 more sources
The newfound relationship between extrachromosomal DNAs and excised signal circles
FEBS Letters, EarlyView.Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley +1 more source