Results 81 to 90 of about 183,908 (336)

Utility of Point-of-Care COVID-19 Testing in an Outpatient Otolaryngology clinic

OTO Open, 2021
Objective To evaluate the utility of point-of-care COVID-19 testing for identifying infected patients in an otolaryngology practice. Study Design Retrospective review of 947 patients tested with a point-of-care nucleic acid amplification test for SARS ...
Meera Ganesh, Craig Cameron Brawley MD, Ashoke Khanwalkar MD, John Mycanka, David B. Conley MD, Robert C. Kern MD, Bruce K. Tan MD, MS   +6 more
doaj   +1 more source

Immunological diagnosis as an adjunctive tool for an early diagnosis of tuberculous meningitis of an immune competent child in a low tuberculosis endemic country: A case report [PDF]

, 2017
Background: Pediatric tuberculous meningitis is a highly morbid, often fatal disease. Its prompt diagnosis and treat - ment saves lives, in fact delays in the initiation of therapy have been associated with high mortality rates. Case presentation:
Ajassa, Camilla, Caraffa, Emanuela, Ciardi, Rosa Maria, Colistra, Davide, DI BIASI, Claudio, Lichtner, Miriam, Mancarella, Cristina, Mascia, Claudia, Mastroianni, Claudio Maria, Mengoni, Fabio, Paglia, Maria Grazia, Vita, Serena, Vullo, Vincenzo   +12 more
core   +4 more sources

International review of blood donation nucleic acid amplification testing

Vox Sanguinis
AbstractBackground and ObjectivesNucleic acid amplification testing (NAT), in blood services context, is used for the detection of viral and parasite nucleic acids to reduce transfusion‐transmitted infections. This project reviewed NAT for screening blood donations globally.Materials and MethodsA survey on NAT usage, developed by the International ...
Helen M. Faddy, Carla Osiowy, Brian Custer, Michael Busch, Susan L. Stramer, Opeyemi Adesina, Thijs van de Laar, Wai‐Chiu Tsoi, Claire Styles, Phil Kiely, Angelo Margaritis, So‐Yong Kwon, Yan Qiu, Xuelian Deng, Antoine Lewin, Signe Winther Jørgensen, Christian Erikstrup, David Juhl, Silvia Sauleda, Bernardo Armando Camacho Rodriguez, Lisbeth Jennifer Catherine Soto Coral, Paula Andrea Gaviria García, Sineenart Oota, Sheila F. O'Brien, Silvano Wendel, Emma Castro, Laura Navarro Pérez, Heli Harvala, Katy Davison, Claire Reynolds, Lisa Jarvis, Piotr Grabarczyk, Aneta Kopacz, Magdalena Łętowska, Niamh O'Flaherty, Fiona Young, Padraig Williams, Lisa Burke, Sze Sze Chua, An Muylaert, Isabel Page, Ann Jones, Christoph Niederhauser, Marion Vermeulen, Syria Laperche, Pierre Gallian, Salam Sawadogo, Masahiro Satake, Ahmad Gharehbaghian, Marcelo Addas‐Carvalho, Sebastián Blanco, Sandra V. Gallego, Axel Seltsam, Marijke Weber‐Schehl, Arwa Z. Al‐Riyami, Khuloud Al Maamari, Fatma Ba Alawi, Hem Chandra Pandey, Dora Mbanya, Rochele Azevedo França, Richard Charlewood   +60 more
openaire   +3 more sources

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Unleashing the Power of Machine Learning in Nanomedicine Formulation Development

Advanced Functional Materials, EarlyView.
A random forest machine learning model is able to make predictions on nanoparticle attributes of different nanomedicines (i.e. lipid nanoparticles, liposomes, or PLGA nanoparticles) based on microfluidic formulation parameters. Machine learning models are based on a database of nanoparticle formulations, and models are able to generate unique solutions
Thomas L. Moore, Cristiano Pesce, Antonietta Greco, Claudia Pisante, Greta Avancini, Valentina Di Francesco, Yosi Shamay, Paolo Decuzzi   +7 more
wiley   +1 more source

Expanding Chemical Space of Nucleic Acid Nanoparticles for Tunable Antiviral‐Like Immunomodulatory Responses and Potent Adjuvant Activity

Advanced Functional Materials, EarlyView.
We introduce a nucleic acid nanoparticle (NANP) platform designed to be rrecognized by the human innate immune system in a regulated manner. By changing chemical composition while maintaining constant architectural parameters, we identify key determinants of immunorecognition enabling the rational design of NANPs with tunable immune activation profiles
Martin Panigaj, Hannah S. Newton, Jian Wang, Laxmi Pandey, Yelixza I. Avilla, Phong Nguyen, Morgan Chandler, Justin Halman, Yasmine Radwan, Stephanie Smith, Elijah Edmondson, Simone Difilippantonio, Chelsea Sanders, Bao Tran, Yongmei Zhao, Shaojun Xie, Edward Cedrone, Barry W. Neun, Meni Wanunu, Nikolay V. Dokholyan, Marina A. Dobrovolskaia, Kirill A. Afonin   +21 more
wiley   +1 more source

Synergistic and non-specific nucleic acid production by T7 RNA polymerase and Bsu DNA polymerase catalyzed by single-stranded polynucleotides

Synthetic and Systems Biotechnology, 2018
Point-of-care molecular diagnostic tests show great promise for providing accurate, timely results in low-infrastructure healthcare settings and at home.
Nicholas J. Emery, Sagardip Majumder, Allen P. Liu   +2 more
doaj   +1 more source

Beating the reaction limits of biosensor sensitivity with dynamic tracking of single binding events [PDF]

, 2018
The clinical need for ultrasensitive molecular analysis has motivated the development of several endpoint-assay technologies capable of single-molecule readout.
Sevenler, Derin, Trueb, Jacob, Unlu, M. Selim   +2 more
core   +2 more sources

Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy

Advanced Functional Materials, EarlyView.
A bimetallic Mn–Ca nanoreactor (MCC) is developed as a non‐nucleotide STING nanoagonist for cancer metalloimmunotherapy. MCC induces Ca2+ overload and hydroxyl radical generation, resulting in mitochondrial damage and mtDNA release. The released mtDNA cooperates with Mn2+ to robustly activate cGAS–STING signaling.
Xin Wang Mo, Nguyen Thi Nguyen, LiLi Guo, Ngoc Man Phan, Hyunsu Shin, Tae‐il Kim, Yu Seok Youn, Jaeyun Kim   +7 more
wiley   +1 more source

Lab-on-PCB with integrated DNA amplification and electroanalytical detection for point-of-care diagnostics

Scientific Reports
Nucleic acid amplification tests (NAATs) are powerful medical diagnostic tools for point-of-care (POC) and other field applications. However, traditional methods like quantitative PCR (qPCR) require complex, expensive equipment and trained operators ...
Seshagopalan Thorapalli Muralidharan, Martin Hanze, Alar Ainla, Björn Möller, Mahiar Max Hamedi, Anna Toldrà   +5 more
doaj   +1 more source