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Research on a Magnetic Separation-Based Rapid Nucleic Acid Extraction System and Its Detection Applications [PDF]
Biosensors, 2023 Nucleic acid extraction represents the “first step” in molecular diagnostic experiments. The quality of this extraction serves as a fundamental prerequisite for ensuring the accuracy of nucleic acid detection. This article presents a comprehensive design
Yao Li +7 more
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Nucleic acid protocols: Extraction and optimization
Biotechnology Reports, 2016 Yield and quality are fundamental features for any researchers during nucleic acid extraction. Here, we describe a simplified, semi-unified, effective, and toxic material free protocol for extracting DNA and RNA from different prokaryotic and eukaryotic ...
Saeed El-Ashram +2 more
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Comparison of the Influence of Different Nucleic Acid Extraction Assays on the Sensitivity of Trypanosoma cruzi-Specific Real-Time PCR [PDF]
Microorganisms, 2022 For the molecular diagnosis of Chagas disease by real-time PCR (polymerase chain reaction), optimization of diagnostic accuracy is desirable. The detection limit of real-time PCR assays for the diagnosis of Trypanosoma cruzi in human serum is affected by
Simone Kann +7 more
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Easy Express Extraction (TripleE)—A Universal, Electricity-Free Nucleic Acid Extraction System for the Lab and the Pen [PDF]
Microorganisms, 2022 The complexity of the current nucleic acid isolation methods limits their use outside of the modern laboratory environment. Here, we describe a fast and affordable method (easy express extraction, called TripleE) as a centrifugation-free and electricity ...
Christian Korthase +3 more
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Biosensors, 2023 Pathogenic pathogens invade the human body through various pathways, causing damage to host cells, tissues, and their functions, ultimately leading to the development of diseases and posing a threat to human health.
Yue Wang +7 more
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Zhongguo gonggong weisheng, 2023 ObjectiveTo establish a sensitive, reliable and rapid pre-treatment method for enrichment and nucleic acid extraction of pathogenic bacteria in water for fluorescent quantitative PCR (qPCR) detection of pathogenic bacteria in water.
Yan WU +4 more
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Pathogens, 2021 This study aimed to assess standard and harsher nucleic acid extraction schemes for diagnostic helminth real-time PCR approaches from stool samples. A standard procedure for nucleic acid extraction from stool and a procedure including bead-beating as ...
Tanja Hoffmann +10 more
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Biosensors, 2022 In this study, an “all-in-one” digital microfluidics (DMF) system was developed for automatic and rapid molecular diagnosis and integrated with magnetic bead-based nucleic acid extraction, loop-mediated isothermal amplification (LAMP), and real-time ...
Siyi Hu +8 more
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Point-of-Care System for HTLV-1 Proviral Load Quantification by Digital Mediator Displacement LAMP
Micromachines, 2021 This paper presents a universal point-of-care system for fully automated quantification of human T-cell lymphotropic virus type 1 (HTLV-1) proviral load, including genomic RNA, based on digital reverse RNA transcription and c-DNA amplification by MD LAMP
Lisa Becherer +22 more
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EVALUATION OF NUCLEIC ACID EXTRACTION METHODS FOR PATHOGEN DETECTION USING PCR [PDF]
Biotechnologia ActaThis work focuses on evaluating nucleic acid extraction methods and optimizing lysis buffer components to enhance nucleic acid yield and minimize the impact of potential inhibitors on target gene amplification to improve the detection of causative ...
D.K. Prokhorenko
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