A rapid and high-yield method for nucleic acid extraction [PDF]
Scientific ReportsWe report a magnetic silica bead-based nucleic acid extraction method which is rapid (6 – 7 min) and efficient (extracts nearly all the nucleic acid in the sample).
Manjula Ramya Vutukuru +4 more
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Dry Swab-Based Nucleic Acid Extraction vs. Spin Column-Based Nucleic Acid Extraction for COVID-19 RT-PCR Testing: A Comparative Study [PDF]
Canadian Journal of Infectious Diseases and Medical Microbiology, 2023 Conventional nucleic acid extraction involves usage of spin columns to isolate the RNA, but this is labor intensive. This study compares the spin column method with a dry swab-based method of extraction using a proteinase K buffer and subsequent heat ...
Mohammed Faraaz Khan, C. Roopa
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Magnetophoresis in Centrifugal Microfluidics at Continuous Rotation for Nucleic Acid Extraction [PDF]
Micromachines, 2022 Centrifugal microfluidics enables fully automated molecular diagnostics at the point-of-need. However, the integration of solid-phase nucleic acid extraction remains a challenge.
Sebastian Hin +6 more
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Method optimization of protein and nucleic acid extraction from skin tape strip samples for biomarker analysis [PDF]
Frontiers in MedicineObjectiveSkin tape stripping is a minimally invasive, skin-specific method for biomarker collection that has gained increasing interest in dermatologic research.
Erika L. Boarder +6 more
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Development and Performance Evaluation of T-prep24: A Novel Automated Nucleic Acid Extraction System Based on Silica Magnetic Beads [PDF]
DiagnosticsBackground: Rapid molecular detection of infectious pathogen with high sensitivity and specificity has become increasingly important in clinical microbiology laboratories.
Jung Ho Park +4 more
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Rapid Equipment-Free Nucleic Acid Extraction Using a Silica-Based Pipet Tip Column. [PDF]
ACS OmegaNucleic acid extraction from clinical specimens is essential for accurate molecular diagnostic assays. However, conventional extraction methods are often slow, equipment-dependent, and require trained personnel, limiting the point-of-care applicability ...
Jung C, Kim G, Song MB, Kim MG.
europepmc +2 more sources
A new approach for detecting HPV DNA in cervical swabs: comparison of nucleic acid extraction with direct PCR [PDF]
Virology JournalBackground Almost all cases of cervical cancer are associated with persistent high-risk HPV infection. WHO prioritizes primary HPV testing for cervical cancer screening.
Fatih Çubuk +6 more
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Comparison of Rapid Nucleic Acid Extraction Methods for SARS-CoV-2 Detection by RT-qPCR [PDF]
Diagnostics, 2022 Since 2020, humanity has been facing the COVID-19 pandemic, a respiratory disease caused by the SARS-CoV-2. The world’s response to pandemic went through the development of diagnostics, vaccines and medicines. Regarding diagnostics, an enormous challenge
Lívia Mara Silva +4 more
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Development and validation of a rapid five-minute nucleic acid extraction method for respiratory viruses [PDF]
Virology JournalBackground The rapid transmission and high pathogenicity of respiratory viruses significantly impact the health of both children and adults. Extracting and detecting their nucleic acid is crucial for disease prevention and treatment strategies.
Yu Wang +8 more
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Improved Nucleic Acid Organic Extraction Through Use of a Unique Gel Barrier Material
BioTechniques, 1996 A novel density barrier material, Phase Lock GelTM, has been formulated and optimized for use in enhancing the separation between aqueous and organic phases during organic extraction/ centrifugation of nucleic acids.
Neil R. Murphy, Randolph J. Hellwig
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