Results 311 to 320 of about 364,067 (350)

Caged molecular beacons: controlling nucleic acid hybridization with light.

Chemical Communications, 2011
We have constructed a novel class of light-activatable caged molecular beacons (cMBs) that are caged by locking two stems with a photo-labile biomolecular interaction or covalent bond.
Chunming Wang, Zhi Zhu, Yanling Song, Hui Lin, C. Yang, W. Tan   +5 more
semanticscholar   +1 more source

Nucleic Acid Hybridization in the Diagnosis of Viral Infections

Clinics in Laboratory Medicine, 1985
Recombinant DNA technology, including molecular cloning and nucleic acid hybridization, is now being applied to problems in clinical virology. Although viral isolation in cell culture remains the most sensitive and specific diagnostic test for many viruses, for some viruses, isolation in cell culture is lengthy or difficult or has not yet been achieved.
Marie L. Landry, Marie L. Landry, Caroline K. Y. Fong   +2 more
openaire   +2 more sources

Synergy of peptide-nucleic-acid and spherical nucleic acid enabled quantitative and specific detection of tumor exosomal microRNA.

Analytical Chemistry, 2019
Exosomal microRNAs are essential in intercellular communications and disease progression, yet it remains challenging to quantify the expression level due to their small size and low abundance in blood.
Li Liu, Hao Lu, Ruixue Shi, Xin-Xin Peng, Qing-wen Xiang, Bo Wang, Qiang-Qiang Wan, Yujie Sun, Fan Yang, Guojun Zhang   +9 more
semanticscholar   +1 more source

Hybridization of Nucleic Acids

1969
I am not sure there is a true difference in DNA-DNA hybridization between DNA from young and old animals. This is a complicated question because of difficulties in preparing DNA completely free of protein, the great complexity of mammalian DNA compared to bacterial DNA, and the greater probability for non specific inter an intra molecular interaction ...
openaire   +2 more sources

A Crosslinked Nucleic Acid Nanogel for Effective siRNA Delivery and Antitumor Therapy.

Angewandte Chemie, 2018
Functional siRNAs are employed as cross-linkers to direct the self-assembly of DNA-grafted polycaprolactone (DNA-g-PCL) brushes to form spherical and nanosized hydrogels via nucleic acid hybridization in which small interfering RNAs (siRNAs) are fully ...
Fei Ding, Quanbing Mou, Yuan Ma, Gaifang Pan, Yuanyuan Guo, Gangsheng Tong, C. H. J. Choi, Xinyuan Zhu, Chuan Zhang   +8 more
semanticscholar   +1 more source

Electrochemical Monitoring of Nucleic Acid Hybridization by Single-Use Graphene Oxide-Based Sensor

, 2011
In this paper, the application of a Graphene oxide (GRPox) integrated on single-use pencil graphite electrode (PGE) for the enhanced monitoring of nucleic acids and for the sensitive and selective detection of the label free DNA hybridization related to ...
M. Muti, Surbhi Sharma, A. Erdem, P. Papakonstantinou   +3 more
semanticscholar   +1 more source

Multiplexed interfacial transduction of nucleic acid hybridization using a single color of immobilized quantum dot donor and two acceptors in fluorescence resonance energy transfer.

Analytical Chemistry, 2010
A multiplexed solid-phase assay for the detection of nucleic acid hybridization was developed on the basis of a single color of immobilized CdSe/ZnS quantum dot (QD) as a donor in fluorescence resonance energy transfer (FRET). This work demonstrated that
W. R. Algar, U. Krull
semanticscholar   +1 more source

Nucleic Acid Hybridization: Triplex Stability and Energetics

Annual Review of Biophysics and Biomolecular Structure, 1995
In this chapter, we review the current state of the thermodynamic database for triple helical oligonucleotide hybridization reactions and present a critical assessment of the methods used to obtain the relevant data. The thermodynamic stability of triple-helix oligonucleotide constructs is discussed in terms of its dependence on temperature, chain ...
S. F. Singleton, Kenneth J. Breslauer, Plum Ge, Daniel S. Pilch   +3 more
openaire   +3 more sources

Microfluidic chip for fast nucleic acid hybridization

Lab on a Chip, 2003
The design and experimental verification of a fast nucleic acid hybridization microchip using the fluidic velocity and strain rate effects was conducted. This hybridization chip was able to increase the hybridization signal 6-fold, reduce non-specific target-probe binding and background noise within 30 min, as compared to conventional hybridization ...
Yung-Chiang Chung, Yu-Cheng Lin, Ming-Zheng Shiu, Wu-Ning Tommy Chang   +3 more
openaire   +3 more sources

Hybridization of nucleic acids directly in agarose gels

Analytical Biochemistry, 1983
Nucleic acids, both DNA and RNA, separated on agarose gels can be visualized by direct hybridization of the dried gel with appropriate radioactive probes. This method does not involve the transfer of the nucleic acid from the gel. The method requires less manipulation than other procedures; it is extremely rapid, sensitive, and inexpensive.
Ronald E. Pearlman, Clifford F. Brunk, Smiley G. S. Tsao   +2 more
openaire   +3 more sources