Results 101 to 110 of about 34,814 (245)

Quantifying Protein–Glycan Interactions Using Native Mass Spectrometry

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Interactions between glycan‐binding proteins (GBPs) and carbohydrates (glycans) are essential to many biological processes relevant to human health and disease. For most GBPs, however, their glycan interactome—the repertoire of glycans recognized and their specificities—is poorly defined.
Duong T. Bui, Elena N. Kitova, Ling Han, Lara K. Mahal, John S. Klassen   +4 more
wiley   +1 more source

Peptide‐Ligand Cooperative Interplay Drives Gold Nanoparticle Encapsulation by Protein Cages

Small, EarlyView.
Gold nanoparticle encapsulation within protein cages is governed by salt‐dependent binding energetics and cooperative peptide–ligand interactions. Ligands enhance electrostatic attraction while peptides extend recruitment and prevent kinetic trapping, enabling robust cargo loading. This molecular‐level insight links experiments and simulations, guiding
Wenhui Li, Niklas Mucke, Michael Rütten, Tommaso L. Schweers, Tobias Beck, Vikram Jadhao   +5 more
wiley   +1 more source

Liposome Particle Size Prediction by In‐Line Process Analytical Technology (PAT)‐Integrated Machine Learning

Small Methods, EarlyView.
An in‐line PAT‐integrated ML strategy for microfluidic liposome synthesis accurately predicted particle size from formulation parameters and fluoresence sensing data. The model captured both seen and unseen conditions, demonstrating robust generalization and process control with low error.
Junghu Lee, Nozomi Morishita Watanabe, Noriko Yoshimoto, Seonghyeon Eom, Moon Kyu Kwak, Ho‐Sup Jung, Hiroshi Umakoshi   +6 more
wiley   +1 more source

The master molecule that built biology: How water shaped the chemistry of life. [PDF]

Protein Sci
Abstract The deep entanglement of biomolecular structure and function with aqueous systems supports the view that water actively sculpted both molecules and processes during the origins of life and continues to constrain evolution today. Nature's rules of biochemistry and biophysics have survived for nearly 4 billion years.
DiGiacomo J, Rose GD, Williams LD.
europepmc   +2 more sources

Prostate cancer diagnosis using plasma extracellular vesicles isolated via aqueous two‐phase systems

VIEW, EarlyView.
Abstract Prostate cancer (PCa) is the second most prevalent cancer in men, with early detection and precise staging remaining critical for improving outcomes. While PSA tests and biopsies are widely used, they suffer from low specificity and often lead to unnecessary procedures.
Yongmin Kwon, Minyeob Lim, Suyeon Kim, Youngju Seo, Meeyoung Kim, Jiyoul Lee, Jaesung Park   +6 more
wiley   +1 more source

The potential for biased signalling in the P2Y receptor family of GPCRs

British Journal of Pharmacology, EarlyView.
The purinergic receptor family is primarily activated by nucleotides, and contains members of both the G protein coupled‐receptor (GPCR) superfamily (P1 and P2Y) and ligand‐gated ion channels (P2X). The P2Y receptors are widely expressed in the human body, and given the ubiquitous nature of nucleotides, purinergic signalling is involved with a plethora
Claudia M. Sisk, Simon Pitchford, Graham Ladds   +2 more
wiley   +1 more source

Thermodynamics of Nucleic Acid Structural Modifications for Biotechnology Applications

, 2011
The repertoire of chemistry available to native nucleotides in DNA and RNA is limited to the purine and pyrimidine functional groups, along with the special role of the 2’-hydroxyl of RNA. The nucleobases have exocyclic amino groups and imines, neither of which is highly reactive or a good candidate for catalytic function.
openaire   +2 more sources

KU80 suppresses endonuclease G activity to preserve genomic integrity

The FEBS Journal, EarlyView.
Under normal conditions, EndoG remains restricted to mitochondria and the genome remains intact. When KU80 is absent, EndoG translocates into the nucleus, where it promotes DNA fragmentation and genomic instability. Thus, this work highlights the importance of KU80 in tightly controlling EndoG localization to preserve genome stability.
Jargalan Batsaikhan, Kwang‐Hyun Park, Hae Ryung Chang, Eunyoung Jung, Myung‐Jin Kim, Jeeyoon Kang, Hyunjo Shim, Eui‐Jeon Woo, Yonghwan Kim   +8 more
wiley   +1 more source

Contrasting effects of different molecular crowding environments on base‐pair opening/closing dynamics of DNA triplex structures

The FEBS Journal, EarlyView.
Base‐pair opening and closing regulate nucleic‐acid structure, stability, and function, but how these motions behave under intracellular molecular crowding remains unclear. Using NMR, we quantified these dynamics in a DNA triplex under two crowder‐reconstituted environments that mimic cellular crowding.
Tomoki Sakamoto, Yudai Yamaoki, Takashi Nagata, Masato Katahira   +3 more
wiley   +1 more source

Aptamer‐mediated outer membrane destabilization overcomes low permeability resistance in Pseudomonas aeruginosa

The FEBS Journal, EarlyView.
DNA aptamers, Apt 60 and Apt 173, target the BamA protein to compromise outer membrane integrity in Pseudomonas aeruginosa, making it susceptible to Azithromycin, an antibiotic that is otherwise poorly effective due to the low‐permeability membrane.
Rupany Selvam, Michelle Khai Khun Yap, Rafael Giacomazzi, Sérgio F. Sousa, Hock Siew Tan   +4 more
wiley   +1 more source