Results 41 to 50 of about 3,808 (162)
RNAi Regulator C3PO Promotes Arbovirus Infection in Insect Vectors
RNA interference (RNAi) is vital for eukaryotes to defend against virus infection. The C3PO complex takes part in RNAi but its role in regulating viral infection remains unclear. This work finds that insect C3PO facilitates arbovirus replication by degrading precursors of miRNAs, especially miR‐971‐3p.
Yan Xiao +7 more
wiley +1 more source
Analysis of the Choristoneura fumiferana nucleopolyhedrovirus genome
The double-stranded DNA genome of Choristoneura fumiferana nucleopolyhedrovirus (CfMNPV) was sequenced and analysed in the context of other group I nucleopolyhedroviruses (NPVs). The genome consists of 129 593 bp with a G+C content of 50·1 mol%. A total of 146 open reading frames (ORFs) of greater than 150 bp, and with no or minimal overlap were ...
Jondavid G, de Jong +6 more
openaire +2 more sources
Identification of miRNAs encoded by Autographa californica nucleopolyhedrovirus
Two Autographa californica nucleopolyhedrovirus (AcMNPV) encoded miRNAs, AcMNPV-miR-1 and AcMNPV-miR-3, have been reported by us in 2013 and 2019, respectively. Here, we present an integrated investigation of AcMNPV-encoded miRNAs, which include the above two miRNAs and three additional newly identified miRNAs.
Jinwen, Wang +9 more
openaire +2 more sources
Phân lập vi rút SENPV từ sâu xanh da láng (Spodoptera exigua Hüber) tại Đồng bằng sông Cửu Long
Thí nghiệm được thực hiện nhằm thu thập và định danh vi rút bằng bằng phương pháp truyền thống dựa trên các triệu chứng và kết hợp với việc thực hiện phản ứng PCR với cặp mồi chuyên biệt PSF002 và PER00 1dùng để nhận biết vi rút nucleopolyhedrovirus (NPV)
Trịnh Thị Xuân +3 more
doaj +1 more source
Synergy of Lepidopteran Nucleopolyhedroviruses AcMNPV and SpliNPV with Insecticides
The joint use of baculoviruses and synthetic insecticides for integrated pest management requires the study of the additive, synergistic or antagonistic effects among them on pest mortality.
Beatriz Dáder +3 more
doaj +1 more source
Abstract The qualified presumption of safety (QPS) process was developed to provide a harmonised safety assessment approach to support EFSA Scientific Panels and Units. The QPS approach assesses the taxonomic identity, body of relevant knowledge and safety concerns of microorganisms intentionally added to the food and feed chain.
EFSA Panel on Biological Hazards (BIOHAZ) +28 more
wiley +1 more source
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) belonging to the group I nucleopolyhedroviruses (NPVs) and expressing the envelope-fusion glycoprotein GP64 transduces a variety of mammalian cells to express foreign genes under the control of mammalian promoters. In contrast, the group II Helicoverpa armigera single NPV (
Changyong, Liang +2 more
openaire +2 more sources
Gene transduction in mammalian cells using Bombyx mori nucleopolyhedrovirus assisted by glycoprotein 64 of Autographa californica multiple nucleopolyhedrovirus [PDF]
AbstractAutographa californica multiple nucleopolyhedrovirus (AcMNPV), an alphabaculovirus, has been widely utilized for protein expression in not only insect cells but also mammalian cells. AcMNPV is closely related to Bombyx mori nucleopolyhedrovirus (BmNPV) and nucleotide sequences of AcMNPV genes have high similarity with those of BmNPV.
Tatsuya Kato +3 more
openaire +2 more sources
Cloning and expression of Antheraea pernyi nucleopolyhedrovirus ptp-2 gene
To elucidate Antheraea pernyi nucleopolyhedrovirus (ApNPV) ptp-2 gene characters, ApNPV ptp-2 gene was cloned using primers based on the sequence of ApNPV PstI-C fragment and then sub-cloned into prokaryotic expression vector pQE-30 to construct ...
SHI Sheng-lin, PAN Min-hui, LU Cheng
doaj +1 more source
A rapidly fabricated hydrogel‐SiO2 composite enables ultralow‐cost, equipment‐minimized DNA extraction, outperforming commercial kits. Hierarchical microstructures enhance hydrophobic/salt‐bridge‐driven adsorption. Integrated with visual LAMP, this field‐deployable system detects Vibrio parahaemolyticus at 10 CFU mL−1 in <40 min, offering ...
Peipei Li +9 more
wiley +1 more source

