Results 51 to 60 of about 5,408 (205)

tRNA nucleotidyltransferase is not essential for Escherichia coli viability. [PDF]

The EMBO Journal, 1987
The role of tRNA nucleotidyltransferase in Escherichia coli has been uncertain because all tRNA genes studied in this organism already encode the -C-C-A sequence. Examination of a cca mutant, originally thought to contain 1-2% enzyme activity, indicated that it actually produces an inactive fragment of 40 kd compared to 47 kd for the wild-type enzyme ...
L. Zhu, Murray P. Deutscher
openaire   +3 more sources

Identification and characterization of the functional tetrameric UDP-glucose pyrophosphorylase from Klebsiella pneumoniae

mBio
In all kingdoms of life, the enzyme uridine diphosphate-glucose pyrophosphorylase (UGP) occupies a central role in metabolism, as its reaction product uridine diphosphate-glucose (UDP-Glc) is involved in various crucial cellular processes.
Isabel Ramón Roth, Pavel Kats, Timm Fiebig, Françoise Routier, Roman Fedorov, Larissa Dirr, Jana I. Führing   +6 more
doaj   +1 more source

Dual targeting of the tRNA nucleotidyltransferase in plants: not just the signal [PDF]

Journal of Experimental Botany, 2007
Enzymes involved in tRNA maturation are essential for cytosolic, mitochondrial, and plastid protein synthesis and are therefore localized to these different compartments of the cell. Interestingly, only one isoform of tRNA nucleotidyltransferase (responsible for adding the 3'-terminal cytidine-cytidine-adenosine to tRNAs) has been identified in plants.
Schmidt von Braun, Serena, Sabetti, Antonino, Hanic-Joyce, Pamela J., Gu, Jun, Schleiff, Enrico, Joyce, Paul B. M.   +5 more
openaire   +5 more sources

Theoretical Studies on Mechanism of Inactivation of Kanamycin A by 4′-O-Nucleotidyltransferase

Frontiers in Chemistry, 2019
This work is focused on mechanistic studies of the transfer of an adenylyl group (Adenoside-5′-monophosfate) from adenosine 5′-triphosphate (ATP) to a OH-4′ hydroxyl group of an antibiotic.
Sergio Martí, Agatha Bastida, Katarzyna Świderek   +2 more
doaj   +1 more source

Mechanism of Mg2+-Accompanied Product Release in Sugar Nucleotidyltransferases [PDF]

Structure, 2018
The nucleotidyl transfer reaction, catalyzed by sugar nucleotidyltransferases (SNTs), is assisted by two active site Mg2+ ions. While studying this reaction using X-ray crystallography, we captured snapshots of the pyrophosphate (product) as it exits along a pocket.
Balaji Prakash, Pravin Kumar Ankush Jagtap, Nisanth N. Nair, Sunil Kumar Verma, Shalini Awasthi, Ravi Tripathi, Neha Vithani   +6 more
openaire   +4 more sources

Proteome Analysis of Corynebacterium diphtheriae–Macrophage Interaction

PROTEOMICS, EarlyView.
ABSTRACT Contact of Corynebacterium diphtheriae with macrophages induces adaptations on both bacterial and cellular sides. The study presented here was aiming to shed light on the simultaneous intracellular adaptation of the bacteria and changes in the proteome of the phagocytes in response to the internalization of C. diphtheriae.
Luca Musella, Jens Möller, Christopher Lischer, Julio Vera‐Gonzalez, Jörg Hofmann, Lisa Ott, Andreas Burkovski   +6 more
wiley   +1 more source

Measuring catalytic mechanism similarity – a new approach to study enzyme function and evolution

The FEBS Journal, EarlyView.
We introduce a previously undescribed computational method to measure the similarity of enzyme mechanisms, enabling comparison of catalytic steps across hundreds of enzymes. Applying this method to a database of enzyme mechanisms demonstrated its ability to automatically uncover functional and evolutionary relationships, independently of sequence and ...
Antonio J. M. Ribeiro, Ioannis G. Riziotis, Neera Borkakoti, Pedro A. Fernandes, Maria J. Ramos, Janet M. Thornton   +5 more
wiley   +1 more source

Structural and Molecular Basis for Resistance to Aminoglycoside Antibiotics by the Adenylyltransferase ANT(2″)-Ia

mBio, 2015
The aminoglycosides are highly effective broad-spectrum antimicrobial agents. However, their efficacy is diminished due to enzyme-mediated covalent modification, which reduces affinity of the drug for the target ribosome.
Georgina Cox, Peter J. Stogios, Alexei Savchenko, Gerard D. Wright   +3 more
doaj   +1 more source

The Molecular Structure and Case Fatality Rate of COVID-19

Translational Research in Urology, 2020
A new coronavirus recognized as 2019 novel coronavirus (COVID-19) is the etiological cause in charge of the 2019-2020 viral pneumonia worldwide outbreak. The molecular structure and genome of this virus are identical to SARA-CoV and for an unknown reason,
Zahra Sadat Aghamir, Sepideh Shivarani , Rima Manafi Shabestari   +2 more
doaj   +1 more source

Nucleotide specificity of the human terminal nucleotidyltransferase Gld2 (TUT2) [PDF]

RNA, 2016
The nontemplated addition of single or multiple nucleotides to RNA transcripts is an efficient means to control RNA stability and processing. Cytoplasmic RNA adenylation and the less well-known uridylation are post-transcriptional mechanisms regulating RNA maturation, activity, and degradation.
David Hyung Suk Jo, Ilka U. Heinemann, Christina Z. Chung   +2 more
openaire   +3 more sources