Results 21 to 30 of about 5,219 (218)
Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci +7 morewiley +1 more sourceScreening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu +9 morewiley +1 more sourceSubspace system identification framework for the analysis of multimoded propagation of THz-transient signals
, 1990 We provide a system identification framework for the analysis of THz-transient data. The subspace identification algorithm for both deterministic and stochastic systems is used to model the time-domain responses of structures under broadband excitation ...O’Donnell, Kane, Birch, David J.S., Xiang, P, Nan Li (155066), Mark A Williams, Chiu, WM, Riis, E., Laursen, Bo Wegge, I H Rodriguez, Martin, Robert W., Yokoyama Toshiyuki, Buric, Michael, C J Hogendoorn, Ling, HY, E N dos Santos, Steenhoven, AA Anton van, ,, Yip, Philip, Jones, J D C, Hossain, Md Moinul, Gardner, J. W., Zhu, HH, To, S, Ooi, A., Alex Attridge, Strain, K. A., Rodrigues, H., Tokmakov, K.V., Gao, X, Huang, QH, G W Baxter, Hogendoorn, C.J., MacPherson, W N, Janina Mazierska, Hunt, Neil T., P K C Wood, Dammalapati, U., Jin, W, Sun, Duo, Hadjiloucas, S., Zhao, D H, Yan, Zhang, Alessandro Salmi, Onuh, S., Rughoobur, Girish, Zhou, X., M J Da Silva, Paolo Minetola, Wang, Shanyuan, Carli L., Chrystie, R., Ju, BF, Mazierska, Janina, Ehmann, KF, Zhang, X, Cheng, L, Karolin, J., Williams, R. J., Zhang, M, Zhang, L, Nguyen, Danh, Zhang, H, Hui Xu (9203), Cree, Michael J., Jaroszynski, D.A., Cheng, X, P C Dastoor, O M H Rodriguez, Flockhart, G M H, Flockhart, Gordon M.H., Griffiths, H., Yong Yan, Sørensen, Thomas Just, Liffman, Kurt, Sidiroglou, Fotios, Birch, David J S, Yan, Yong, Guan, BO, Flewitt, AJ, Yin, JH, Xu, Hui, Williams, Mark A, Levi, R, Zhou, Kaiming, Dick, A.R., Cantatore, Angela, Choi, PC, Flewitt, Andrew J., Kaminski, C.F., Wynne, Klaas, Chen, Tong, Sutalo, Iiija, Carli, Lorenzo, Nand, Anbhawa, Pennella, Francesco, Yu, Tongxi, Yu, Winnie, Williams, M. A. (Mark A.), Guofeng Qiao (16049912), R Levi, Zhou Zhou (154603), Morbiducci U., Barbato, Giulio, Stewart, M., Yang, SM, Wood, Paul, Huang, Qing-Hua, F Sidiroglou, Flockhart, Gordon, Srinivasan, G., Flockhart, G, G Barbato, Gardner, JW, Harris, J., Walsh, Martin A., Bowen, J. W., , E., Thomas, Sanju, Jacob, M.V., Schiavi, A., Burns, I.S., Chen, Xianfeng, Md. Moinul Hossain, Perrone, Guido, David J S Birch, Maguire, L., Dong, Ming, Gardner, Julian W., Robb, G.R.M., Lau, Kin Tak, ROTINAT, René, Maklad, Mokhtar, Aldham, B., , F., Swinehart, Philip R, Bal, Harpreet Kaur, Tokmakov, Kirill, Cranch, G.A., Luca Iuliano, Chow, KW, Xu, DS, McLoskey, D., Li, Yi; id_orcid, Oishi, S., Yang, M, Xu, Di, MacPherson, William N.; id_orcid, Walker, Keith, Kaminski, C., Judd, Martin D., Tran, Y., Gao, Wei, Yang, Y, Tucker NP, Stewart, Martin, Hall, M., Lim, NY, Ren, MJ, Tosi, D., Becerra, Victor M., Genta, Gianfranco, McKenna, M., Chen, Zhong-Ping, Wang, SY, Krupka, Jerzy, Durst, F., Tucker, Nicholas P., McCartney, N., A A Van Steenhoven, Huang, Yan-Ping, 3156, 3155, Laursen, Bo W, Hoskisson, Paul A., Fan, S, Hua, T, Wu, YS, Borkowski, M., Barr, M., Popov, Ivan, Costa, N., Lee, WB, Barbosa, C., Fan, J, Zhang, Hui, Candelaresi M, Yang, Li, Li, Nan, Zheng, YP, 陈曦, L Carli, 3159, Whitenett, Gillian, 3158, 3157, Qiu, Huihe, Wang, Xiaoru, Zhou, Zhou, Ng, Sun-pui, 王小如, Hariharan, P., Ling, Hang Yin, Galvao, R. K. H., Fan, JT, Stewart, George, Lange, de, H.C., Takaya, Yasuhiro, Fernandes, C., Yu, T, Attridge, Alex, Baxter, Gregory W, Yu, W, Hourahine, B., Dotchev, Krassimir, Wang, Wei, ZHOU, Wei, HAMPEL, U., Strain, K.A., Villa-López, Farah-Helúe, Jones, J. D C, Hogendoorn, CJ Jankees, Apperson, Kathleen, Chow, Kwok Wing, Huihe Qiu, Hult, J., O'Hagan, W.J., Wan, X, Gao, Yongsheng, Fung, EHK, Li, Qingmin, , M., Araki Tsutomu, Kong, LB, 10324328, Parker, Anthony W., Demokan, MS, Guglielmone C., Kitcher, Daniel J, Danh Nguyen, Jamison, S.P., Xu, BG, P Hariharany, S F Collins, OMINI M., Robb, Kirsty, Guglielmone, C., G Genta, Xishi Wang, Ho, HL, Genta, G, H C De Lange, Dorrington, Adrian A., Tam, HY, Steenhoven, van, A.A., Varum, H., Jia, W., Parker AW, Levi, Raffaello, Xiao, J, Chen, Kevin P., Sagoo, K., McNeil, B.W.J., Lockerbie, Nicholas, Walsh MA, Hsu, C. T., Cheng, KW, K M O'Donnell, Xuan, HF, Esposito, E., Qiao, Guofeng, Galvao, Roberto K. H., O'Donnell, Kane, Bowen, John W., Tao, X, O'Connor, D., Wang, Shu-Zhe, Pennella F, S A Wade, Wang, Xishi, Hong, Fangjun, Salmi, Alessandro, Villa-López, Farah Helue, Carnegie, Dale A., Cain, M. G., Pei, HF, Lees, K., Ozawa, Y., Wang, H, Piner, Chris, Iuliano, Luca, Wang, L, Yoshimori Kyu, Wade, Scott A, Wang, S, Duan, W, Gough, W., Zhu, WL, MOULART, Raphaël, Li, David D U, Collins, Stephen F, Maier, R R J, Cheng, Li, Bennion, I, Yu, Hongbo, Becerra, V. M., Birch, David J. S., Krupka, J., Larmour, I. A., Robb K, Zheng, Yong-Ping, Payne, Andrew D., Knight, Alex E, Conroy, Richard M., Birch, David, Xin, Lingling, W Allison, Cantatore, A., De Chiffre L., Aoshima, S., Adamczyk, Katrin, Dastoor, P., Rolinski, Olaf J., Hong, CY, Zhu, Z, Candelaresi, Marco, He, YH, Culshaw, B., Z Brodzeli, Tao, XM, Fahy, A., Norris, I., He, Yong-Hong, Muniz-Piniella, A., De Chiffre, Leonardo, U Hampel, Lange, HC Rick de, Knight, Alex E., Li, J, Matsuda, K., Moodie, David, Li, H, Rosenberg, Martin, YAMAMOTO, Fujio, Li, C, Flockhart, G. M H, Watson, S., Hadjiloucas, Sillas, Guo, Guangmei, NISHIUMI, Toyohiko, McGuiness, C.D., Keating, M., Barton, J S, Wang, Xudong, SILVA, M. J. Da, SUZUKI, Takao, Kong, L, Cain, M., Olivero, Massimo, L De Chiffre, Infield, D.G., X J Zhou, Ng, SP, Karolin, Jan, Feng, J, Thomas, S, Nguyen, Thinh B, Li, Y, Johnstone, Walter, Qiu, H. H., Gumiero, Andrea, De Chiffre, L., Moinul Hossain, Md, McConnell, G., Cheng, Xiaoyin, Saito, Takayuki, Tao, Xiaoming, Kirkendall, C.K., Lu, MH, Morbiducci, Umberto, Birch, D.J.S., Choy, CL, Strigazzi, Alfredo, Birch, D. J. S., Zhao, Donghui, Maier, R. R J; id_orcid, Krystek, Michael, Pate, R.C., RODRIGUEZ, I. H., MacGregor, S.J., Thomas, O., Sommerfeld, M., Gang Lu, Zhang, Lin, Ferreira, L. A., Stewart, Hazel L, Manasseh, R., Chen, X F, Easton, C.D., Jerzy Krupka, Lau, KT, Rolinski, O.J., D J S Birch, Araújo, F. M., Huang, YP, Siew, W.H., Illesinghe, S., H K Bal, Qian, X, Ren, M, 91c9be23-1416-4160-ad4e-accfee1b658a, Papoff, F., Guo, T, Cole, Marina, Wei Wang (17594), Kumar, Jagadeesha, A Fahy, GUGLIELMONE, CLAUDIO LUIGI DOME, Yokoyama Shuko, Chen, ZP, Stewart , Hazel L., Carli, L., Guo, Y, Burns, D., SANTOS, E. N. dos, Hossain, Md. Moinul, Hegarty, T., Jayadevaiah, M., David Day-Uei Li (3954032), Chan, HLW, Vopson, Melvin, Chen, Xi, Sutter, Jens, Brodzeli, Zourab, Stanton, T., A Cantatore, Wiggins, Mark, Chen, YS, Chrystie, Robin S.M., Kar, F, Villa-López, FH, M Barr, Wang, DN, Lu, Gang, Stewart, G., Jagadeesha Kumar, Hoskisson PA, Chen, Y., Jones, Rhys, Barbato, G, Geddes, C.D., Graham, D., Adamczyk K, Wood, P. K. C., Costa, A., Bennion, Ian, Gumiero A, Rughoobur, G, Jones, D.R., Cheung, Amy, Minetola, Paolo, Cheung, CF, Sherrington, D.C., Choi, KF, Thomsen, L., Barton, James S., Yip , Philip, Atherton, K., Faulds, K., Omini, M., Lakshminarayanan, V., Thompson, S., Liao, Y, Sarkar, M, Schiavi A., Zhou, K M, Lockerbie, N.A., Burns, I., Sparavigna, Amelia Carolina, SCHIAVI, ALESSANDRO, Allison, W., Hunt NT, Cantatore A., Marshall, Joanna, RODRIGUEZ, O. M. H., Cole, M +484 morecore +1 more sourceIdentifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky +7 morewiley +1 more sourcePharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling
FEBS Open Bio, EarlyView.Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx +5 morewiley +1 more sourceRoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano +2 morewiley +1 more source
