Results 121 to 130 of about 8,353,130 (346)

Impact of aging on the pathophysiology of dry eye disease: A systematic review and meta-analysis

, 2022
Purpose Dry eye disease (DED) is a common age-related ocular surface disease. However, it is unknown how aging influences the ocular surface microenvironment.
Hughes, JWB, Bozza, N, Shih, KC, Yokoi, N, Sotozono, C, Numa, K, Kitazawa, K, Tomioka, Y, Campisi, J, Sana, R, Inomata, T   +10 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Clinical Correlations of Novel Autoantibodies in Patients with Dry Eye

Journal of Immunology Research, 2019
Background. Diagnostic criteria for Sjögren’s syndrome (SS) are continually being updated in pursuit of more precise and earlier diagnosis to prevent its complications.
Sezen Karakus, Alan N. Baer, Esen K. Akpek   +2 more
doaj   +1 more source

Clinical Evidence on Allogeneic Blood-Derived Eye Drops in Ocular Surface and Corneal Diseases

PurposeTo systematically review clinical studies on allogeneic blood-derived eye drops in ocular surface and corneal diseases.MethodsA PRISMA-guided search of PubMed, Google Scholar, Scopus, Web of Science, and Embase (1985–September 2025) identified ...
Ghalibafan, Seyyedehfatemeh, Sabater, Alfonso L, John, Ansu Ann, Djalilian, Ali R   +3 more
core   +1 more source

A multicenter report of the use of plasma rich in growth factors (PRGF) for the treatment of patients with ocular surface diseases in North America

, 2022
To investigate the efficacy and safety of plasma rich in growth factors (PRGF) eyedrops in the management of patients with ocular surface diseases in North America. Multicenter interventional case series of patients using PRGF eyedrops for the first time.
Pflugfelder, Stephen C., Soifer, Matias, Yennam, Sowmya, Wang, Margaret, Mousa, Hazem M., Perez, Victor L., Tovar, Arianna, Sabater, Alfonso L.   +7 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Novel Approaches for Imaging-Based Diagnosis of Ocular Surface Disease

, 2020
Imaging has become indispensable in the diagnosis and management of diseases in the posterior part of the eye. In recent years, imaging techniques for the anterior segment are also gaining importance and are nowadays routinely used in clinical practice ...
Leopold Schmetterer, Schlatter, Andreas, Bingyao Tan, Gerhard Garhöfer, Tan, Bingyao, Jacqueline Chua, Chua, Jacqueline, Doreen Schmidl, Schmetterer, Leopold, Garhöfer, Gerhard, Andreas Schlatter, Schmidl, Doreen   +11 more
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Cyclosporine in ocular surface inflammation [PDF]

Eye, 2017
Dry eye disease (DED) is the most frequent encountered ocular surface condition and remains one of the world’s most overlooked and treatment elusive ocular conditions. There a multitude of lubricating eye drops, agents/approaches to deal with the condition and associated external eye disease. Without gaining much in symptomatic relief.
openaire   +3 more sources

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source