Results 101 to 110 of about 4,998,430 (314)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Characterising the ocular surface and tear film in a population-based birth cohort of 45-year old New Zealand men and women.

PurposeTo assess the prevalence of dry eye disease, aqueous tear deficiency, meibomian gland dysfunction, and asymptomatic ocular surface disease in a population-based cohort of 45-year-old New Zealand men and women.MethodsThis cross-sectional study of ...
Wilson, Graham A, Craig, Jennifer P, Cheyne, Kirsten, Ambler, Antony, Wang, Michael TM   +4 more
core   +1 more source

Evaluation of the Performance of an Ocular Surface Modulator Containing ST-Lysyal Versus Hyaluronic Acid Eyedrops in Patients with Dry Eye Disease: A Pilot Study

Ophthalmology and Therapy
Introduction Dry eye disease (DED) is a multifactorial condition of the ocular surface, primarily treated with tear substitutes, which do not fully restore natural tear functions.
Stefano Barabino, Andrea Rosa, Sara Marini, Carlo Domenico Bianchi, Maurizio Rolando   +4 more
doaj   +1 more source

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

FEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph, Andrea B. Pham, Kiara E. Bornes, Razan H. Hammad, Chelsea A. Rousseve‐Ross, Thomas M. Huckaba, Terry J. Watt, Tasha B. Toro   +7 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Anatomy of cornea and ocular surface

Indian Journal of Ophthalmology, 2018
Important functions of cornea in the eye include protecting the structures inside the eye, contributing to the refractive power of the eye, and focusing light rays on the retina with minimum scatter and optical degradation. Considerable advances have taken place in understanding the organization of collagen in the corneal stroma and its clinical ...
openaire   +3 more sources

Innate immunity of the ocular surface

Brain Research Bulletin, 2010
The ocular surface epithelium serves a critical function as the defensive front line of the innate immune system. While the detection of microbes is arguably its most important task, an exaggerated host defense reaction to endogenous bacterial flora may initiate and perpetuate inflammatory mucosal responses.
Shigeru, Kinoshita, Mayumi, Ueta
openaire   +3 more sources

Single cell analysis of short-term dry eye induced changes in cornea immune cell populations

Frontiers in Medicine
BackgroundDry eye causes corneal inflammation, epitheliopathy and sensorineural changes. This study evaluates the hypothesis that dry eye alters the percentages and transcriptional profiles of immune cell populations in the cornea.MethodsDesiccating ...
Jehan Alam, Ebru Yaman, Gerda Cristal Villalba Silva, Rui Chen, Cintia S. de Paiva, Mary Ann Stepp, Stephen C. Pflugfelder   +6 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Vector analysis valuation of astigmatism in the cornea after femtosecond laser-assisted corneal astigmatism incision in cataract surgery

BMC Ophthalmology
Background The aim of this article is to evaluate the effectiveness of anterior penetrating femtosecond laser-assisted astigmatism keratotomy (FS-AK) using vector analysis.
Dan Yang, Shifeng Fang, Yi Yang, Ning Bao, Pin Zuo, Shujie Miao, Xinxia Xue, Jixin Zou   +7 more
doaj   +1 more source