Results 171 to 180 of about 17,527 (209)

Deswapping bovine odorant binding protein

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2008
The X-ray structure of bovine Odorant Binding Protein (bOBP) revealed its association as a domain swapped dimer. bOBP, devoid of any cysteines, contrasts with other mammalian OBPs, which are monomeric and possess at least one disulfide bridge. We have produced a mutant of bOBP in which a glycine residue was inserted after position 121.
RAMONI, Roberto, SPINELLI S, GROLLI, Stefano, CONTI, Virna, MERLI, Elisa, CAMBILLAU C, TEGONI M.   +6 more
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Odorant-binding proteins of the mouse

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 1995
After the isolation of two odorant-binding proteins (OBP-I and OBP-II) from mouse nasal tissue, we have purified two additional OBPs, which bind tritiated 2-isobutyl-3-methoxypyrazine. OBP-III is a homodimer with subunits of M(r) 22,000 and pI 4.2. OBP-IV is a homodimer with subunits of M(r) 21,000 and pI 4.85.
PES D, PELOSI, PAOLO
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Odorant‐Binding Proteins: Structural Aspects

Annals of the New York Academy of Sciences, 1998
ABSTRACT: Structural data on odorant‐binding proteins (OBPs), both in vertebrates and in insects, are reviewed and discussed. OBPs are soluble proteins interacting with odor molecules and Pheromones in the perireceptor areas, the nasal mucus in vertebrates and the sensillar lymph in insects.
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The molecular identification, odor binding characterization, and immunolocalization of odorant-binding proteins in Liriomyza trifolii

Pesticide Biochemistry and Physiology, 2022
The Liriomyza trifolii is a highly invasive polyphagia pest. Understanding the physiological functions of odorant binding proteins (OBPs) in the chemical communication of L. trifolii can lead to effective pest management strategies. Seven full-length OBPs were identified by transcriptome screening of L. trifolii adults.
Qikai, Zhang, Zibo, Li, Dongkai, Chen, Shengyong, Wu, Haihong, Wang, Yunlong, Li, Zhongren, Lei   +6 more
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DyBOBS: A Dynamic Biomimetic Assay for Odorant-Binding to Odor-Binding Protein

Chemosensory Perception, 2010
An in vitro system was developed to mimic the structural and flow conditions of the human olfactory epithelium and to measure the dynamics of odorant-binding to odor-binding protein (OBP). A hydrophilic fused silica capillary, coated internally with a thin (about 1.3 µm) aqueous film of recombinant rat-OBP3 mimicked the human olfactory epithelium ...
Yabuki, Masayuki, Portman, Katherine, Scott, David, Briand, Loïc, Taylor, Andrew   +4 more
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Odorant binding proteins of Heliothis virescens

Insect Biochemistry and Molecular Biology, 1993
cDNA clones coding for three different binding proteins were isolated from an antennal library of Heliothis virescens. The deduced amino acid sequences showed only moderate homology to each other but shared several common structural features. Based on a comparison with the predicted primary structures of antennal binding proteins from different moth ...
J, Krieger, H, Gänssle, K, Raming, H, Breer   +3 more
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Electrochemical biosensing with odorant binding proteins

2020
The development of sensors that mimic the natural smell sensing mechanism and selectively recognizes the odorants remains highly challenging. Electrochemical based sensing approaches aiming at monitoring molecular recognition events between surface receptors and analytes in solution or in the gas phase, are one possible transduction platforms among ...
Szunerits, Sabine, Boukherroub, Rabah, Vasilescu, Alina   +2 more
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Odorant Binding to Bovine Odorant Binding Protein Detected by Intrinsic Fluorescence

Chemistry Letters, 2005
Abstract Odorant binding to bovine odorant binding protein (OBPb) made OBPb structure a little tighter and odorant detection by intrinsic fluorescence possible. For odorant with high affinity such as 3,7-dimethyl-1-octanol (DMO), the binding reaction proceeded in a two-step manner that inferred an existence of a third binding site.
Mineo Ikematsu, Daizo Takaoka, Masashi Yasuda   +2 more
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Odorant binding initially occurring at the central pocket in bovine odorant-binding protein

Biochemical and Biophysical Research Communications, 2005
Why bovine odorant-binding protein (OBPb), among OBP family, assumes a dimeric structure has been unclear. Here we clarified, by measuring the fluorescence of intrinsic tryptophan and tyrosine residues of intact OBPb and OBPb whose C-terminal 10 amino acids were deleted, that odorant enters the central pocket formed by the dimerization when OBPb first ...
Mineo, Ikematsu, Daizo, Takaoka, Masashi, Yasuda   +2 more
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Immunocytochemistry of Odorant-Binding Proteins

1994
Odorant-binding proteins are small water-soluble proteins that have been detected in the perireceptor compartment of olfactory receptor cells of vertebrates and insects [1,2]. Although their definite physiological role in olfaction is still unclear, similar functions have been proposed in stimulus transport or inactivation [1–6].
Steinbrecht, R., Laue, M., Zhang, S., Ziegelberger, G.   +3 more
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