Results 301 to 310 of about 1,424,575 (357)

In situ hybridization with oligonucleotide probes

2002
Publisher Summary In situ hybridization (ISH) is an important method for tracing the regional and cellular sites of gene expression (mRNA distribution) within a tissue. This method comes into its own when applied to the intermingled cell types of the brain.
William Wisden, Brian J. Morris
openaire   +3 more sources

An oligonucleotide probe specific for Onchocerca volvulus

Molecular and Biochemical Parasitology, 1989
A genomic DNA library of a Liberian strain of Onchocerca volvulus was prepared in the vector bacteriophage lambda gt10. The library was differentially screened by hybridisation with radiolabelled total DNA from the homologous parasite, two heterologous Onchocerca parasites (Onchocerca gibsoni and Onchocerca gutturosa) and human liver cells.
William Harnett, Alfons Renz, R. Michael E. Parkhouse, Anne E. Chambers   +3 more
openaire   +3 more sources

Optimized oligonucleotide probes for DNA fingerprinting

ELECTROPHORESIS, 1988
AbstractThe three different simple repetitive oligonucleotide probes (CT)8, (CAC)5 and (TCC)5 were hybridized to a panel of human DNAs which had been digested with the restriction endonucleases Alu I, Hinf I and Mbo I. The resulting DNA fingerprints were analyzed and different parameters calculated, such as the maximal mean allele frequency and the ...
Hans Zischler, Jörg T. Epplen, Renate Schäfer, Andrea Becker, Uli Birsner   +4 more
openaire   +3 more sources

Probe Design Strategies for Oligonucleotide Microarrays

2016
Oligonucleotide microarrays have been widely used for gene detection and/or quantification of gene expression in various samples ranging from a single organism to a complex microbial assemblage. The success of a microarray experiment, however, strongly relies on the quality of designed probes.
Parisot, Nicolas, Peyretaillade, Eric, Dugat-Bony, Eric, Denonfoux, Jérémie, Mahul, Antoine, Peyret, Pierre   +5 more
openaire   +5 more sources

Triplex Tethered Oligonucleotide Probes

Journal of the American Chemical Society, 1996
RNA molecules are believed to possess highly complex structures containing singleand double-stranded regions as well as loops, bulges, and pseudoknots.1 This structural complexity severely complicates efforts to design structureand/or sequenceselective RNA-binding molecules.2,3 Recently we described a family of molecules for the sequenceand structure ...
Alanna Schepartz, Arikha C. Moses
openaire   +2 more sources

ChemInform Abstract: Chemically Modified Oligonucleotides as Probes and Inhibitors [PDF]

ChemInform, 1991
AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Gauss Dh, Uwe Englisch
openaire   +2 more sources

Oligonucleotide probes for DNA fingerprinting in horses

Journal of Animal Breeding and Genetics, 1993
Summary10 different oligonucleotide probes were evaluated for DNA fingerprinting in horses. Five probes were able to detect polymorphic bands. The probes (GT)8, (GTG)5 and (GGAT)4 are most informative for individual identification and were used to analyze a population of Hannoveranian horses.
Hermann Geldermann, K. Wilke, M. Weimann, M. Jung   +3 more
openaire   +3 more sources

Oligonucleotide probes functionalization of nanogap electrodes

ELECTROPHORESIS, 2017
AbstractNanogap electrodes have attracted a lot of consideration as promising platform for molecular electronic and biomolecules detection. This is mainly for their higher aspect ratio, and because their electrical properties are easily accessed by current‐voltage measurements. Nevertheless, application of standard current‐voltages measurements used to
Rosa Letizia Zaffino, Mònica Mir, Josep Samitier   +2 more
openaire   +3 more sources

Development of an oligonucleotide probe specific for Trichophytonrubrum

British Journal of Dermatology, 1999
A species-specific DNA probe was developed to detect the dermatophyte species Trichophyton rubrum. The selected oligonucleotide sequence is derived from the highly variable internal transcribed spacer 2 region of the ribosomal DNA operon. The specificity of the non-radioactive labelled oligonucleotide probe was tested against related dermatophytes ...
Wolfram Sterry, Wolfgang Presber, M. El Fari, Yvonne Gräser, H.-J. Tietz   +4 more
openaire   +3 more sources

Oligonucleotide probes for DNA fingerprinting in dogs

Journal of Animal Breeding and Genetics, 1994
SummaryTen different oligonucleotide probes were evaluated for DNA fingerprinting in dogs. Seven probes are able to detect polymorphic bands. Probes (GT)8, (GTG)5 and (GGAT)4 are most informative for individual identification. The probabilities that two individuals from different breeds have the same DNA fingerprint pattern are 1.7 × 10−7, 5.5 × 10−8 ...
M, Jung, K, Wilke, H, Geldermann
openaire   +2 more sources