Results 71 to 80 of about 222,321 (300)

Developmental and tissue-specific expression of the Q5k gene [PDF]

, 1991
Expression of the Q5k gene was examined by northern blot analysis and polymerase chain reaction (PCR) in the AKR mouse and various cell lines, each of the H-2k haplotype.
Baeuerle, Patrick A., Bevec, Dorian, Brem, Gottfried, Schwemmle, Sabine, Urban, Manuela B., Weiss, Elisabeth H.   +5 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Large Scale Explorative Oligonucleotide Probe Selection for Thousands of Genetic Groups on a Computing Grid: Application to Phylogenetic Probe Design Using a Curated Small Subunit Ribosomal RNA Gene Database

The Scientific World Journal, 2014
Phylogenetic Oligonucleotide Arrays (POAs) were recently adapted for studying the huge microbial communities in a flexible and easy-to-use way. POA coupled with the use of explorative probes to detect the unknown part is now one of the most powerful ...
Faouzi Jaziri, Eric Peyretaillade, Mohieddine Missaoui, Nicolas Parisot, Sébastien Cipière, Jérémie Denonfoux, Antoine Mahul, Pierre Peyret, David R. C. Hill   +8 more
doaj   +1 more source

Spotted cotton oligonucleotide microarrays for gene expression analysis

BMC Genomics, 2007
Background Microarrays offer a powerful tool for diverse applications plant biology and crop improvement. Recently, two comprehensive assemblies of cotton ESTs were constructed based on three Gossypium species.
Nettleton Dan, Gingle Alan R, Lee Jinsuk J, Swanson Jordan M, Rapp Ryan A, Hovav Ran, Woodward Andrew W, Cheung Foo, Flagel Lex E, Udall Joshua A, Town Christopher D, Chen Z Jeffrey, Wendel Jonathan F   +12 more
doaj   +1 more source

The T-cell oncogenic protein HOX11 activates Aldh1 expression in NIH 3T3 cells but represses its expression in mouse spleen development [PDF]

, 1998
Hox11 is a homeobox gene essential for spleen formation in mice, since atrophy of the anlage of a developing spleen occurs in early embryonic development in Hox11 null mice.
Greene, W.K., Bahn, S., Masson, N., Rabbitts, T.H.   +3 more
core   +2 more sources

Empirical Establishment of Oligonucleotide Probe Design Criteria [PDF]

Applied and Environmental Microbiology, 2005
ABSTRACT Criteria for the design of gene-specific and group-specific oligonucleotide probes were established experimentally via an oligonucleotide array that contained perfect match (PM) and mismatch probes (50-mers and 70-mers) based upon four genes.
Zhili, He, Liyou, Wu, Xingyuan, Li, Matthew W, Fields, Jizhong, Zhou   +4 more
openaire   +2 more sources

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

The application of species specific DNA-targeted probes and fluorescently tagged lectin to differentiate several species of Pseudo-nitzschia (Bacillophyceae) in Chinhae Bay, Korea

Scientia Marina, 2001
We applied molecular methods using a DNA probe and fluorescently tagged lectins to discriminate toxic Pseudo-nitzschia multiseries from the Chinhae Bay for a Korean harmfulalgae monitoring program. From the binding activity of lectins, P. multistriata, P.
Eun Seob Cho, Jong Gyu Park, Bong Cheol Oh, Yong Chul Cho   +3 more
doaj   +1 more source

A strategy for oligonucleotide microarray probe reduction [PDF]

Genome Biology, 2002
One of the factors limiting the number of genes that can be analyzed on high-density oligonucleotide arrays is that each transcript is probed by multiple oligonucleotide probes. To reduce the number of probes required for each gene, a systematic approach to choosing the most representative probes is needed. A method is presented for reducing the number
Antipova, Alena A., Tamayo, Pablo, Golub, Todd R.   +2 more
openaire   +3 more sources