Results 91 to 100 of about 222,321 (300)

Molecular probes for the identification of avian Haemoproteus and Leucocytozoon parasites in tissue sections by chromogenic in situ hybridization

open access: yesParasites & Vectors, 2019
Background Avian haemosporidian parasites can cause severe disease in their hosts due to excessive exo-erythrocytic merogony and anaemia caused by blood stages.
Tanja Himmel   +8 more
doaj   +1 more source

The HLA-E Gene encodes two differentially regulated Transcripts and a Cell Surface Protein [PDF]

open access: yes, 1992
An HLA-E-specific oligonucleotide probe was used to study the expressioonf HLA-E. This probed etects two HLA-E transcripts, 1.8 and 2.7 kb in size, which are present in varying ratios in allt issues and cell lines investigated.
Honka, Thomas   +4 more
core   +1 more source

Selective and Precise Editing of Digital Polymers Through Parallel or Series Toehold‐Mediated Strand Displacement

open access: yesAdvanced Functional Materials, EarlyView.
A sequence‐encoded supramolecular construct containing two accessible toeholds is developed herein for enabling multiple editing operations. By introducing specific input strands, it is possible to selectively erase or rewrite digital content through parallel or series toehold‐mediated strand displacement (PTMSD or STMSD).
Jakub Ossowski   +3 more
wiley   +1 more source

Identification of Chromosomes and Chromosome Rearrangements in Crop Brassicas and Raphanus sativus: A Cytogenetic Toolkit Using Synthesized Massive Oligonucleotide Libraries

open access: yesFrontiers in Plant Science, 2020
Crop brassicas include three diploid [Brassica rapa (AA; 2n = 2x = 16), B. nigra (BB; 2n = 2x = 18), and B. oleracea (CC; 2n = 2x = 20)] and three derived allotetraploid species.
Neha Agrawal   +5 more
doaj   +1 more source

DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus [PDF]

open access: yes, 1995
We used three short repetitive nucleotide sequences [(GTG)5, (TAC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted Citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic ...
Asif, M   +7 more
core   +2 more sources

DNA‐Templated 2D Heterostructures as Phototriggered Dynamic Nanohybrids: From Releasing Molecular Loads to Controlling Enzyme Biocatalytic Function

open access: yesAdvanced Functional Materials, EarlyView.
DNA strands are employed both as dynamic linkers and nanoscale templates for the integration of Ag2S nanoparticles on MoS2, which in turn imparted photothermal responsiveness; this feature permits the selective cargo (fluorophore, quantum dots or an enzyme) release from the MoS2 surface in response to local heat induced by light irradiation.
Kai Chen   +3 more
wiley   +1 more source

Molecular biological methods for studying the gut microbiota : the EU human gut flora project [PDF]

open access: yes
Seven European laboratories co-operated in a joint project (FAIR CT97-3035) to develop, refine and apply molecular methods towards facilitating elucidation of the complex composition of the human intestinal microflora and to devise robust methodologies ...
Blaut, M.   +5 more
core   +2 more sources

VIVID: A qPCR‐Based Platform for Sensitive and Quantitative In Vivo Tracking of Extracellular Vesicles

open access: yesAdvanced Functional Materials, EarlyView.
This study introduces VIVID (Vesicle In Vivo Identification using DNA), a qPCR‐based platform that tracks PCR‐amplifiable DNA tags loaded in the EVs for accurate and quantifiable EV biodistribution in vivo. ABSTRACT Extracellular vesicles (EVs) represent promising carriers for nucleic acid therapeutics, offering advantages over synthetic nanoparticles ...
Oscar Boyadjian   +5 more
wiley   +1 more source

Fluorogenic PNA probes

open access: yesBeilstein Journal of Organic Chemistry, 2018
Fluorogenic oligonucleotide probes that can produce a change in fluorescence signal upon binding to specific biomolecular targets, including nucleic acids as well as non-nucleic acid targets, such as proteins and small molecules, have applications in ...
Tirayut Vilaivan
doaj   +1 more source

Nonradioactive method to detect native single-stranded G-tails on yeast telomeres using a modified Southern blot protocol

open access: yesBioTechniques, 2011
Because of their low abundance and short length, telomeric single-stranded extensions have not traditionally been assessed by Southern blot analysis.
Lina M. Ortega   +2 more
doaj   +1 more source

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