Results 81 to 90 of about 312,210 (300)

Sub-Nanomolar Detection of Oligonucleotides Using Molecular Beacons Immobilized on Lightguiding Nanowires

Nanomaterials
The detection of oligonucleotides is a central step in many biomedical investigations. The most commonly used methods for detecting oligonucleotides often require concentration and amplification before detection.
Therese B. Johansson, Rubina Davtyan, Julia Valderas-Gutiérrez, Adrian Gonzalez Rodriguez, Björn Agnarsson, Roberto Munita, Thoas Fioretos, Henrik Lilljebjörn, Heiner Linke, Fredrik Höök, Christelle N. Prinz   +10 more
doaj   +1 more source

Palladium-Catalyzed Modification of Unprotected Nucleosides, Nucleotides, and Oligonucleotides

Molecules, 2015
Synthetic modification of nucleoside structures provides access to molecules of interest as pharmaceuticals, biochemical probes, and models to study diseases.
Kevin H. Shaughnessy
doaj   +1 more source

Oligonucleotides suppress PKB/Akt and act as superinductors of apoptosis in human keratinocytes

, 2009
DNA oligonucleotides (ODN) applied to an organism are known to modulate the innate and adaptive immune system. Previous studies showed that a CpG-containing ODN (CpG-1-PTO) and interestingly, also a non-CpG-containing ODN (nCpG- 5-PTO) suppress ...
Annette Dorn, Diamant Thaçi, Hofmann, Matthias, Roland Kaufmann, Andreas Bock, Maike Schultz, Thaçi, Diamant, Stefan Kippenberger, Bock, Andreas, Schultz, Maike, Dorn, Annette, Hüseyin Aygün, Kippenberger, Stefan, August Bernd, Matthias Hofmann, Aygün, Hüseyin, Bernd, August, Müller, Jutta, Kaufmann, Roland, Jutta Müller   +19 more
core   +1 more source

Kelvin Probe Force Microscopy in Bionanotechnology: Current Advances and Future Perspectives

Advanced Materials, EarlyView.
Kelvin probe force microscopy (KPFM) enables the nanoscale mapping of electrostatic surface potentials. While widely applied in materials science, its use in biological systems remains emerging. This review presents recent advances in KPFM applied to biological samples and provides a critical perspective on current limitations and future directions for
Ehsan Rahimi, Mario Palacios‐Corella, Arjan Mol, Salvador Pané, Josep Puigmartí‐Luis   +4 more
wiley   +1 more source

Nanocomposites consisting of titanium dioxide nanoparticles, antisense oligonucleotides, and photoactive groups as agents for effective action on nucleic acids

Журнал микробиологии, эпидемиологии и иммунобиологии
Relevance. Studies on model systems have confirmed the effectiveness of antisense oligonucleotides, including those that contain photoactive groups, for the modification of nucleic acids.
Asya S. Levina, Marina N. Repkova, Oleg Y. Mazurkov, Elena V. Makarevich, Natalya A. Mazurkova, Valentina F. Zarytova   +5 more
doaj   +1 more source

Viral Infection‐Inspired Autonomous Detection of Fusion‐Competent Viruses for Screening and Environmental Surveillance

Advanced Materials, EarlyView.
Inspired by viral entry mechanisms, the FUSION assay enables autonomous detection of respiratory viruses via membrane fusion–triggered CRISPR‐Cas13a activation. VEACON selectively fuses with fusion‐competent viruses, triggering fluorescence within confined vesicles.
Jae Chul Park, Yidam Song, Hyun‐Woo Choi, Jaeuk Sung, Harin Jin, WonSeok Gwak, Kirim Yoo, Soohwang Lee, Jongeon Park, Jeongmin Kim, Hye‐Jun Jo, Jahyun Koo, Youngdo Jeong, Kwan Hyi Lee, Seung‐Jung Kee, Hojun Kim   +15 more
wiley   +1 more source

Synthesis and properties of new fluorescent dyes and dye-labelled oligonucleotides

, 2012
Fluorescence is widely used in DNA sequencing and genetic analysis. Novel fluorescence techniques for studying nucleic acids and other biologically important molecules are constantly being developed.
Gerowska, Marta
core  

Stable Protein‐Based G‐Quadruplex‐Derived Supramolecular Bioinks as Tunable ECM‐Mimetic Constructs Assembled by Combining Non‐Covalent and Covalent Strategies

Advanced Materials, EarlyView.
Harnessing the synergistic interplay of supramolecular self‐assembly, under macromolecular crowding conditions, and enzymatic‐mediated covalent crosslinking toward a stable protein‐based G‐quadruplex‐derived supramolecular bioink. This bioinspired strategy enables the biofabrication of complex and tunable ECM‐mimetic constructs, providing a platform ...
Vera Sousa, Rita Sobreiro‐Almeida, Bart W. L. van den Bersselaar, E. W. Meijer, Ghislaine Vantomme, João Borges, João F. Mano   +6 more
wiley   +1 more source

Development of Novel antimiRzymes for Targeted Inhibition of miR-21 Expression in Solid Cancer Cells

Molecules, 2019
MicroRNAs (miRNAs) are short non-coding RNAs that are involved in the regulation of gene expression. Previous reports showed an over-expression of miRNA-21 (miR-21) in various cancer cells, and its up-regulation is closely related to cancer initiation ...
Leon M. Larcher, Tao Wang, Rakesh N. Veedu   +2 more
doaj   +1 more source

Polymerase-endonuclease amplification reaction for large-scale enzymatic production of antisense oligonucleotide

, 2009
Synthetic oligonucleotides are contaminated with highly homologous failure sequences. Oligonucleotide synthesis is difficult to scale up because it requires expensive equipments, hazardous chemicals, and tedious purification process.
Deming Gou, Xiaolong Wang
core