Results 71 to 80 of about 323,621 (299)

Nanopore-based massively parallel sensing for peptide profiling and protein identification

Nature Communications
Nanopore-based single-molecule sensing holds immense promise for revolutionizing proteomics, however, its practical application remains constrained by low throughput, suboptimal library preparation, and limited analytical power for complex, stochastic ...
Ji Wang, Junyi Chen, Hailin Pan, Fengqin Luo, Wenbing Qin, Huixian Zeng, Xilong Yuan, Yuchen Qiao, Yunfeng Zhang, Yishuo Zhang, Dapeng Wang, Liang Shen, Zhiwei Zhai, Qianhua Zhu, Yuqing Deng, Xiaojing Sheng, Qingqing Xie, Yuning Zhang, Xu Yan, Tao Zeng, Mengzhe Shen, Yinqi Bai, Bo Teng, Yuxiang LI, Chuanyu Liu, Ou Wang, Yuliang Dong, Siqi Liu, Xun Xu   +28 more
doaj   +1 more source

An Efficient Nitroblue Tetrazolium Staining and Bright-Field Microscopy Based Method for Detecting and Quantifying Intracellular Reactive Oxygen Species in Oocytes, Cumulus Cells and Embryos

Frontiers in Cell and Developmental Biology, 2020
Assessment of intracellular reactive oxygen species (ROS) is important for evaluating the developmental ability of cumulus-oocyte complexes (COC) and embryos.
Pradeep K. Javvaji, Pradeep K. Javvaji, Arindam Dhali, Joseph R. Francis, Joseph R. Francis, Atul P. Kolte, Anjumoni Mech, Sudhir C. Roy, Ashish Mishra, Raghavendra Bhatta   +9 more
doaj   +1 more source

Five‐Year Disease Progression in Synuclein Seeding Positive Sporadic Parkinson's Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To provide a comprehensive description of disease progression in synuclein seeding assay (SAA) positive sporadic Parkinson Disease participants, using Neuronal Synuclein Disease integrated biological and functional impairment staging framework.
Paulina Gonzalez‐Latapi, Caroline Gochanour, Seung Ho Choi, Hyunkeun Cho, Chelsea Caspell‐Garcia, Christopher Coffey, Michael Brumm, David‐Erick Lafontant, Yuge Xiao, Thomas Tropea, John Seibyl, Caroline Tanner, Charles S. Venuto, Karl Kieburtz, Lana M. Chahine, Kathleen L. Poston, Andrew Siderowf, Kenneth Marek, Tanya Simuni, The Parkinson's Progression Markers Initiative   +19 more
wiley   +1 more source

Development of FuGO: An ontology for functional genomics investigations [PDF]

, 2006
The development of the Functional Genomics Investigation Ontology (FuGO) is a collaborative, international effort that will provide a resource for annotating functional genomics investigations, including the study design, protocols and instrumentation ...
Brinkman, Ryan R., Causton, Helen C., Fan, Liju, Field, Dawn, Fostel, Jennifer, Fragoso, Gilberto, Gray, Tanya, Heiskana, Mervi, Hernandez-Boussard, Tina, Smith, Barry, Whetzel, Patricia L.   +10 more
core  

Ketogenic Diet as an Epigenetic Therapy in SETD1B‐Related Epilepsy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Histone lysine methyltransferases such as SETD1B regulate chromatin structure and gene transcription. Ketone bodies, including butyrate, act as histone deacetylase inhibitors. We report a 4‐year‐old boy with SETD1B‐related absence epilepsy, refractory to conventional medications, who achieved sustained > 90% seizure reduction on the Modified ...
Erica Tsang, Brian S. Gloss, Jessica P. Hayes, Andrew J. A. Holland, Manoj P. Menezes, Joceline A. Branson, Shekeeb S. Mohammad, Jingya J. Yan, Shrujna Patel, Velda X. Han, Russell C. Dale   +10 more
wiley   +1 more source

Advancements in Multiple Myeloma Research: High-Throughput Sequencing Technologies, Omics, and the Role of Artificial Intelligence

Biology
Multiple myeloma is a complex and challenging type of blood cancer that affects plasma cells in the bone marrow. In recent years, the development of advanced research techniques, such as omics approaches—which involve studying large sets of biological ...
Alejandra Gutiérrez-González, Irene Del Hierro, Ariel Ernesto Cariaga-Martínez   +2 more
doaj   +1 more source

Tunable fractionation of nucleic acids

BioTechniques, 2009
We developed a method for selective purification of DNA using the cationic detergent, cetyltrimethylammonium bromide (CTAB), accompanied with urea and controlled high-salt (NaCl) concentration.
Md. Salimullah, Sachiko Kato, Mitsuyoshi Murata, Chika Kawazu, Charles Plessy, Piero Carninci   +5 more
doaj   +1 more source

RNA Sequencing Resolves Cryptic Pathogenic Variants in Mitochondrial Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Mitochondrial diseases are the most common inherited metabolic disorders, characterized by pronounced clinical and genetic heterogeneity that complicates molecular diagnosis. Although DNA‐based sequencing approaches have become standard in genetic testing, up to half of patients remain without a definitive diagnosis.
Zhimei Liu, Xin Duan, Fatemeh Peymani, Jia Wang, Chengjia Bao, Chaolong Xu, Ying Zou, Zixuan Zhang, Yunxi Zhang, Tongyue Li, Martin Pavlov, Junling Wang, Minhan Song, Tianyu Song, Xiaodi Han, Mingxi Sun, Danmin Shen, Ruoyu Duan, Huafang Jiang, Manting Xu, Holger Prokisch, Fang Fang   +21 more
wiley   +1 more source

Whole-genome profiling of age- and sex-associated DNA methylation signatures in human plasma cell-free DNA

Communications Medicine
Background Age and sex significantly impact DNA methylation patterns, however, existing datasets typically include only a subset of methylation sites in the human genome, hindering our thorough understanding.
Wei Chen, Jinjin Xu, Guodan Zeng, Rijing Ou, Changlin Yang, Chuang Xu, Yeqin Wang, Xinxin Wang, Qiuyan Li, Chenhui Zhao, Wenwen Zhou, Yu Lin, Wending Pang, Haiqiang Zhang, Jianhua Yin, Yan Zhang, Xin Jin   +16 more
doaj   +1 more source

CAR T‐Cell Therapy in Neurology: A Scoping Review of Neuro‐Oncology, Autoimmune Diseases & Neurotoxicity

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Chimeric antigen receptor (CAR) T‐cell therapy has been investigated in neurological diseases, encompassing both central nervous system malignancies and autoimmune disorders, thereby extending its application beyond hematological cancers.
Omar Alqaisi, Mohammed Dibas, Patricia Tai, Ala Dibas, Osama Souied, Suhair Al‐Ghabeesh   +5 more
wiley   +1 more source