Results 171 to 180 of about 2,155,238 (316)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

On-line tool condition monitoring using artificial neural networks

, 1996
On-line tool condition monitoring using artificial neural ...
Guy Littlefair (13071435), D Adradi (13734922), A Hope (13734919), M Javed (13586149), B Rao (13716235), G Smith (9093014)   +5 more
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

A Novel Raman-Chromatography Assembly for Automated Calibration and In-Line Monitoring in Bioprocessing. [PDF]

Eng Life Sci
Heyer-Müller J, Schiemer R, Lopinski M, Wang C, Willems F, Robbel L, Schmitt M, Hubbuch J.   +7 more
europepmc   +1 more source

The Microcellular Structure of Injection Molded Thick-Walled Parts as Observed by In-Line Monitoring. [PDF]

Materials (Basel), 2020
Sykutera D, Czyżewski P, Szewczykowski P.   +2 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

In-Line Monitoring of Milk Lactose for Evaluating Metabolic and Physiological Status in Early-Lactation Dairy Cows. [PDF]

Life (Basel)
Girdauskaitė A, Arlauskaitė S, Rutkauskas A, Džermeikaitė K, Krištolaitytė J, Televičius M, Malašauskienė D, Anskienė L, Japertas S, Antanaitis R.   +9 more
europepmc   +1 more source

Cracking the code Monitoring the International Code of Marketing of Breast-milk Substitutes

, 1997
Available from British Library Document Supply Centre- DSC:02/41332 / BLDSC - British Library Document Supply CentreSIGLEGBUnited ...
Interagency Group on Breastfeeding Monitoring (United Kingdom)
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source