FEBS Open Bio, EarlyView.Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
The genetically encoded biosensor HyPer7 enables in-line monitoring of H2O2 accumulation dynamics in the methylotrophic yeast Komagataella phaffii. [PDF]
FEMS Yeast ResMendes Honorato V +3 more
europepmc +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Application of an Escherichia coli triple reporter strain for at-line monitoring of single-cell physiology during L-phenylalanine production. [PDF]
Eng Life Sci, 2023 Hoang MD +5 more
europepmc +1 more source
, 2007 Wei N. A machine vision-based in situ probe for automated on-line measurement of cell density and viability by dark field microscopy, image processing and pattern recognition.
Wei, Ning
core
FEBS Open Bio, EarlyView.5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley +1 more source
A Self-Configurable BUS Network Topology Based on LoRa Nodes for the Transmission of Data and Alarm Messages in Power Line-Monitoring Systems. [PDF]
Sensors (Basel)Alorda-Ladaria B, Pons M, Isern E.
europepmc +1 more source
In-line monitoring of latex-particle size during emulsion polymerizations with a high polymer content of more than 60. [PDF]
RSC Adv, 2020 Jacob LI, Pauer W.
europepmc +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source