Results 161 to 170 of about 17,928,993 (286)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Relationships between farmer well-being and the welfare of their animals: A One Welfare scoping review. [PDF]

Anim Welf
Levallois P, Buczinski S, Desmarchelier M, Lupien S, Robichaud MV.   +4 more
europepmc   +1 more source

WSAVA Animal Welfare Guidelines for companion animal practitioners and veterinary teams [PDF]

, 2019
Bacon, H., Endenburg, N., Seksel, K., Lee, N., Ryan, S., Takashima, G., Jouppi, R., dASS BW-2, Hazel, S., Human Animal Relationships / One Welfare   +9 more
core   +2 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Enhancing Laboratory Animal Welfare through Motivational Training of Facility Personnel: A One Welfare Approach. [PDF]

J Am Assoc Lab Anim Sci
Franzoni Ribeiro B, Gomes de Macedo Braga LM, Zaqueu de Lima T, Alves Santos LG, Speck da Silva ML, Barbosa Fialho Martins V, de Souza Munhoz LL, Reginato Facciotti P, Dos Santos Martins D, de Azevedo Ruiz VL.   +9 more
europepmc   +1 more source

Animal welfare, human welfare. Putting one welfare in pig production into perspective

Human welfare and animal welfare and their environment are interdependent, leading to a new multidisciplinary scientific approach and the building of a global movement around these societal issues. In France, the RMT "One Welfare", a joint technology network, is working to put this concept into practice at the farm level.
Ramonet, Yannick, Boivin, Xavier
openaire   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

A one welfare perspective on calf health: a qualitative study of knowledge, attitudes, working conditions, working atmosphere, and communication among farmers and calf-care teams on large Saxon dairy farms. [PDF]

Front Vet Sci
Douay-Ryckelynck M, Retzler P, Meier KK, Merle R, Müller KE, Stock A, Jensen KC.   +6 more
europepmc   +1 more source