Results 91 to 100 of about 132,905 (308)

Expression and localization of nitric oxide synthase isoforms during porcine oocyte growth and acquisition of meiotic competence

Czech Journal of Animal Science, 2009
Reproduction biotechnologies depend on the use of fully meiotically competent oocytes. Growing oocytes without full meiotic competence are an interesting potential source due to their quantity, but the mechanisms regulating the processes of acquisition ...
E. Chmelíková, M. Sedmíková, J. Petr, T. Kott, V. Lánská, L. Tůmová, H. Tichovská, M. Ješeta   +7 more
doaj   +1 more source

NAD+ Metabolism Licenses Zygotic Genome Activation via PARP7‐Mediated ADP‐Ribosylation of UHRF1 in Mouse Early Embryos

Advanced Science, EarlyView.
This study uncovers a metabolic‐epigenetic axis licensing zygotic genome activation (ZGA) in mouse embryos. A developmental decline in NAD+ levels activates PARP7, which mono‐ADP‐ribosylates and stabilizes UHRF1. This modification promotes the establishment of permissive histone acetylation marks, thereby facilitating timely ZGA.
Guangyi Cao, Ziqi Zhang, Anqi Chen, Yanbo Liu, Yuling Lin, Lina Yu, Ruixin Shi, Aolei Guo, Yan Mao, Ganggui Lou, Chaojun Li, Luhong Wen, Guijun Yan, Haixiang Sun   +13 more
wiley   +1 more source

EFNA5 as an Oocyte-Derived Factor Enhances Developmental Competence by Modulating Oxidative Stress, Inflammation, and Apoptosis During In Vitro Maturation

Antioxidants
In vitro maturation (IVM) of oocytes remains suboptimal due to oxidative stress and disrupted cumulus–oocyte communication. Oocyte-derived factors (ODFs) are key mediators of this crosstalk and crucial for oocyte competence.
Xingyuan Liu, Jian Cui, Yubing Wang, Jia Hao, Yingjie Wu, Yinjuan Wang, Lei An, Jianhui Tian, Guangyin Xi   +8 more
doaj   +1 more source

Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor

Czech Journal of Animal Science, 2009
Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor SNAP (2mM). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure.
T. Krejčová, J. Petr, M. Krejčová, K. Kheilová   +3 more
doaj   +1 more source

Biallelic Germline Inactivation of HROB Causes Primary Gonadal Insufficiency and is Potentially Associated with Colonic Polyposis Predisposition

American Journal of Medical Genetics Part A, EarlyView.
ABSTRACT The Homologous Recombination Factor With OB‐Fold (HROB) plays a role in homologous recombination and DNA replication, where it enhances the MCM8‐MCM9 helicase complex activity. Recent findings link biallelic germline HROB variants to primary gonadal insufficiency (hypergonadotropic hypogonadism), a phenotype also associated with MCM8/MCM9 ...
Noah C. Helderman, Carli M. Tops, Jelmer Legebeke, Ting Yang, Marcos Díaz Gay, Diantha Terlouw, Lisa E. E. L. O. Lashley, Stefan Aretz, Anna K. Sommer, Mariona Terradas, Laura Valle, Richarda M. de Voer, Ludmil B. Alexandrov, Hans Morreau, Tom van Wezel, Maartje Nielsen   +15 more
wiley   +1 more source

Aberrant cortex contractions impact mammalian oocyte quality

The cortex controls cell shape. In mouse oocytes, the cortex thickens in an Arp2/3-complex-dependent manner, ensuring chromosome positioning and segregation.
Verlhac, Marie-Hélène, de Labbey, Ghislain, Nikalayevich, Elvira, Shihabi, Anastasia, Turlier, Hervé, Terret, Marie-Emilie, Innocenti, Metello, Letort, Gaëlle, Yahiatene, Mohamed, Schuh, Melina, Todisco, Elena, Voituriez, Raphaël, Pollet-Villard, Xavier   +12 more
core   +3 more sources

A simple method for assessing hyaluronic acid production by cumulus-oocyte complexes

Czech Journal of Animal Science, 2016
The cumulus expansion of cumulus-oocyte complex (COC) is an essential regulating process of oocyte maturation and as such it is a possible biomarker of the in vitro maturing oocytes quality.
K. Zámostná, J. Nevoral, T. Kott, R. Procházka, M. Orsák, M. Šulc, V. Pajkošová, V. Pavlík, T. Žalmanová, K. Hošková, F. Jílek, P. Klein   +11 more
doaj   +1 more source

De Novo 2.2 Mb 19q13.42–q13.43 Microdeletion Encompassing U2AF2: Support for a Haploinsufficiency Model

American Journal of Medical Genetics Part A, EarlyView.
ABSTRACT U2 small nuclear RNA auxiliary factor 2 (U2AF2) is an essential pre‐mRNA splicing factor involved in the early stages of pre‐mRNA splicing. To date, multiple individuals have been reported with predominantly heterozygous missense variants presenting intellectual disability, speech and motor delays, seizures, hypotonia, and thin or hypoplastic ...
Amanda Toledo, Sarah Araji, Weimin Bi, Seema R. Lalani   +3 more
wiley   +1 more source

Exocyst complex component 1 (Exoc1) loss in dormant oocyte disrupts c-KIT and growth differentiation factor (GDF9) subcellular localization and causes female infertility in mice

Cell Death Discovery
A limited number of female germ cells support reproduction in many mammals. The follicle, composed of oocytes and supporting granulosa cells, forms the basis of oogenesis.
Chi Lieu Kim Nguyen, Yumeno Kuba, Hoai Thu Le, Hossam Hassan Shawki, Natsuki Mikami, Madoka Aoki, Nanako Yasuhara, Hayate Suzuki, Saori Mizuno-Iijima, Shinya Ayabe, Yuki Osawa, Tomoyuki Fujiyama, Tra Thi Huong Dinh, Miyuki Ishida, Yoko Daitoku, Yoko Tanimoto, Kazuya Murata, Woojin Kang, Masatsugu Ema, Yuji Hirao, Atsuo Ogura, Satoru Takahashi, Fumihiro Sugiyama, Seiya Mizuno   +23 more
doaj   +1 more source

Zebrafish and CRISPR—A synergistic approach to decipher and cure human diseases

Animal Models and Experimental Medicine, EarlyView.
Zebrafish, with high genetic homology to humans, serves as a powerful vertebrate model for disease modeling and drug discovery. Integration of CRISPR/Cas9 technology enables precise genome editing, facilitating the development of translational models for human diseases.
Manikandan Sivaprakasam, Aashika Raagavi Jeanpierre, Salma Mohammed, Rajesh Srinivasan, Aman Kumar Mohanty   +4 more
wiley   +1 more source