Results 171 to 180 of about 16,809,928 (387)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

open access: yesFEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Parental acceptance of HPV vaccinations at ages 9–10 in the United States

open access: yesHuman Vaccines & Immunotherapeutics
In the US, HPV vaccination is routinely recommended for 11–12-y-olds. This study explored parental attitudes toward initiation of the HPV vaccine at 9–10 y of age. Parents of 9–12-y-old children in the U.S.
Kunal Saxena   +5 more
doaj   +1 more source

Open access publishing: What is world’s best practice? [PDF]

open access: yes, 2013
Open access publishing delivers on the dream of centuries - the free and unfettered access to the written word. However there are shades of openness and the question raised is 'what is world's best practice for open access publishing?' Four criteria are
Paull, John
core  

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

open access: yesFEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino   +7 more
wiley   +1 more source

Potential acceptance of HPV vaccination at ages 9–10 in the United States among parents of HPV vaccine-naïve children

open access: yesHuman Vaccines & Immunotherapeutics
This study explored attitudes/perceptions among parents who have not yet agreed to HPV vaccination for their 11–12-y-old children. US parents/guardians were recruited from a consumer panel for this online, cross-sectional survey.
Kunal Saxena   +5 more
doaj   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

open access: yesFEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue   +6 more
wiley   +1 more source

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