Results 221 to 230 of about 991,153 (346)

Increasing the pneumonia treatment coverage among children under 5 years old through 'Enhanced Management of Pneumonia in the Community': implementation research protocol. [PDF]

BMJ Open
Nsona H, Golombe E, Zulu U, Magombo C, Twaibu H, Mbemba P, Msowoya C, Chagoma E, Sadala A, Dedza C, Mangwilisa A, Banda R, Chizani N, Mpezeni W, Qazi SA, Nisar YB.   +15 more
europepmc   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Establishing One Health Surveillance Platform for Electronic Integrated Disease Surveillance and Response in Malawi: Action Design Research Study. [PDF]

JMIR Public Health Surveill
Wu TJ, Mvula MV, Chado EKK, Kamanga BN, Mapemba DD, Billy RE, Nyirongo L, Mwale AC, Banda EC, Kagoli M, Manda TD, Bjune GA, Kaasbøll JJ.   +12 more
europepmc   +1 more source

Qualitative evaluation of integrated employment and skills trials: implementation report [PDF]

, 2009
Francis, Ruth, Hillage, Jim, Johnson, Claire, Levesley, Tom, Oakley, Joy, Sissons, Paul   +5 more
core  

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

WHO's structured process for creating health-conducive environments in countries: insights and examples from the African region. [PDF]

Glob Health Action
Wolf J, Vert-Rocca C, Anya BM, Mariama Baissa A, Gordon B, Bertram M, Lasuba CLP, Neira M, Onyango AW, Tomas BA, Prüss A.   +10 more
europepmc   +1 more source

Mapping research into the delivery of work-based learning [PDF]

, 2002
Hughes, Maria, Turner, Paul
core  

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source