Results 41 to 50 of about 146,124 (285)

Activation of Silent gal Genes in the lac-gal Regulon of Streptococcus thermophilus [PDF]

, 2001
Streptococcus thermophilus strain CNRZ 302 is unable to ferment galactose, neither that generated intracellularly by lactose hydrolysis nor the free sugar.
Vos, W.M., de, Kuipers, Oscar P.; id_orcid, Kuipers, Oscar P., van den Bogaard, P.T.C., Catzeddu, Pasquale,, Bogaard, Patrick T.C. van den, Vaughan, Elaine E., Bogaard, P.T.C., van den, Vaughan, E.E., Kuipers, Oscar P.,, Bogaard, Patrick T.C. van den,, Kuipers, O.P., Vaughan, Elaine E.,, Vos, Willem M. de, Vos, Willem M. de,, de Vos, W.M., Catzeddu, Pasquale, Catzeddu, P.   +17 more
core   +2 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis

mBio
We describe a new Chlamydia trachomatis protein depletion method that uses an engineered small RNA (sRNA) to inhibit translation of a target gene. In proof-of-principle experiments, we induced functional knockdown of IncA, a fusion-mediating inclusion ...
Janina Ehses, Kevin Wang, Asha Densi, Cuper Ramirez, Ming Tan, Christine Sütterlin   +5 more
doaj   +1 more source

Horizontal gene transfer and diverse functional constrains within a common replication-partitioning system in Alphaproteobacteria: the repABC operon

BMC Genomics, 2009
Background The repABC plasmid family, which is extensively present within Alphaproteobacteria, and some secondary chromosomes of the Rhizobiales have the particular feature that all the elements involved in replication and partitioning reside within one ...
Cevallos Miguel A, González Víctor, Vázquez-Castellanos Jorge F, Castillo-Ramírez Santiago   +3 more
doaj   +1 more source

The non-classical ArsR-family repressor PyeR (PA4354) modulates biofilm formation in Pseudomonas aeruginosa [PDF]

, 2012
PyeR (PA4354) is a novel member of the ArsR family of transcriptional regulators and modulates biofilm formation in Pseudomonas aeruginosa. Characterization of this regulator showed that it has negative autoregulatory properties and binds to a ...
Plower, E., Mooij, M., McCarthy, R., Wang, Y. P., Dobson, A., O'Gara, F., O'Gara, F., Mac Aogáin, M., Tian, Z.X., Morrissey, J., Wang, Y.P., Tian, Z., Adams, C., McCarthy, R.R., McCarthy, R. R., Wang, Y., Tian, Z. X., O'Gara, Fergal, Mooij, M.J., Mooij, M. J.   +19 more
core   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Relatively slow stochastic gene-state switching in the presence of positive feedback significantly broadens the region of bimodality through stabilizing the uninduced phenotypic state. [PDF]

PLoS Computational Biology, 2018
Within an isogenic population, even in the same extracellular environment, individual cells can exhibit various phenotypic states. The exact role of stochastic gene-state switching regulating the transition among these phenotypic states in a single cell ...
Hao Ge, Pingping Wu, Hong Qian, Xiaoliang Sunney Xie   +3 more
doaj   +1 more source

Operon and non-operon gene clusters in the C. elegans genome [PDF]

WormBook, 2014
Nearly 15% of the ~20,000 C. elegans genes are contained in operons, multigene clusters controlled by a single promoter. The vast majority of these are of a type where the genes in the cluster are ~100 bp apart and the pre-mRNA is processed by 3' end formation accompanied by trans-splicing.
Thomas, Blumenthal, Paul, Davis, Alfonso, Garrido-Lecca   +2 more
openaire   +2 more sources

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source

N-acetylgalatosamine-mediated regulation of the aga operon by AgaR in Streptococcus pneumoniae

Frontiers in Cellular and Infection Microbiology, 2016
Here, we analyze the transcriptomic response of Streptococcus pneumoniae D39 to N-acetylgalactosamine (NAGa). Transcriptome comparison of S. pneumoniae D39 grown NAGaM17 (0.5% NAGa + M17) to that grown in GM17 (0.5% Glucose + M17) revealed the elevated ...
Muhammad Afzal, Muhammad Afzal, Sulman Shafeeq, Hifza Ahmed, Oscar P. Kuipers   +4 more
doaj   +1 more source