Results 111 to 120 of about 146,400 (289)

Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Animal Research and One Health, EarlyView.
Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley   +1 more source

Microarray analysis of the ler regulon in enteropathogenic and enterohaemorrhagic escherichia coli strains [PDF]

, 2014
The type III protein secretion system is an important pathogenicity factor of enteropathogenic and enterohaemorrhagic Escherichia coli pathotypes. The genes encoding this apparatus are located on a pathogenicity island (the locus of enterocyte effacement)
Islam, Md. Shahidul, Islam, Shahidul, Mala Patel, Patel, Mala, Pallen, Mark J,, Busby, Stephen J W; id_orcid, Penn, Charles W, Pallen, Mark J., Bingle, Lewis, Charles W Penn, Lewis E H Bingle, Penn, C. W., Shaw, Robert K, Md Shahidul Islam, Lee, David J., Snyder, Lori A. S., Islam, Md Shahidul, Bingle, Lewis E. H., Busby Stephen J. W., Lori A S Snyder, Snyder Lori A. S., Busby, Stephen J. W., Constantinidou Chrystala, Mark J Pallen, Constantinidou, Chrystala, Snyder, Lori A S, Lee, David J, Penn Charles W., Pallen, Mark J, Islam Md. Shahidul, David J Lee, Robert K Shaw, Patel Mala, Bingle, Lewis E H, Chrystala Constantinidou, Stephen J W Busby, Penn, Charles W., Pallen Mark J., Lee David J., Bingle Lewis E. H., Shaw Robert K., Busby, Stephen J W, Shaw, Robert K.   +42 more
core   +1 more source

The Nuclear Receptor Testicular Receptor 4 Orchestrates Cytoskeletal Organization in a G Protein Subunit Alpha 12/Rho‐Associated Protein Kinase–Dependent Manner to Promote Myofibroblast Differentiation and Tissue Fibrosis in Systemic Sclerosis

Arthritis &Rheumatology, EarlyView.
Objective Members of the superfamily of nuclear receptors have been implicated in inflammatory processes and pathologic tissue remodeling and have emerged as attractive targets for pharmaceutical intervention. However, the role of testicular receptor 4 (TR4; or Nr2c2) in fibroblast activation and rheumatologic diseases has not yet been investigated ...
Yun Zhang, Lichong Shen, Junhui Zheng, Gisela Baur, Dilidaer Yasen, Honglin Zhu, Yi‐Nan Li, Xianyi Meng, Thuong Trinh‐Minh, Tim Filla, Debomita Chakraborty, Andrea‐Hermina Györfi, Christina Bergmann, Adam Reich, Alexander Kreuter, Georg Schett, Jörg H. W. Distler   +16 more
wiley   +1 more source

Expression and Activity of the BioH Esterase of Biotin Synthesis is Independent of Genome Context

Scientific Reports, 2017
BioH is an α/β-hydrolase required for synthesis of the pimelate moiety of biotin in diverse bacteria. The bioH gene is found in different genomic contexts.
Xinyun Cao, Lei Zhu, Zhe Hu, John E. Cronan   +3 more
doaj   +1 more source

Transcriptome dynamics-based operon prediction in prokaryotes [PDF]

, 2014
Background Inferring operon maps is crucial to understanding the regulatory networks of prokaryotic genomes. Recently, RNA-seq based transcriptome studies revealed that in many bacterial species the operon structure vary with the change ...
Smolander, Olli-Pekka, Fortino, Vittorio, Olli-Pekka Smolander, Dario Greco, Roberto Tagliaferri, Auvinen, Petri, Petri Auvinen, Greco, Dario, Olli Pekka Smolander, Tagliaferri, Roberto, Vittorio Fortino   +10 more
core   +1 more source

Enhancing the Secretion Systems: Genetic Engineering of Super Bioagents for Effective Plant Disease Control

Biotechnology and Bioengineering, EarlyView.
Genetic engineering (GE) overcomes BCA limits, enabling robust, scalable crop protection. “Super Bioagents” (SBs) use optimized SSs to enhance bioactive molecule delivery. Optimizing SSs enhance precise, sustainable effector deployment for stable disease suppression. SBs use microbiome‐informed design for scalable, next‐gen sustainable crop protection.
Michael Dare Asemoloye
wiley   +1 more source

Identification of the Integration/Excision Module and Regulatory Elements Involved in the Mobility of IME8, an Integrative and Mobilizable Element From Mosquitocidal Lysinibacillus sphaericus

Microbial Biotechnology
Lysinibacillus sphaericus, a bacterium successfully used in the control of mosquitoes, bears its insecticidal traits in GI8, a recently identified mosquitocidal genomic island.
Yifeng Hu, Ying Yang, Xiaomin Hu, Jacques Mahillon, Zubin Chen, Han Xia   +5 more
doaj   +1 more source

Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes

, 2018
Summary Operon-mapper is a web server that accurately, easily and directly predicts the operons of any bacterial or archaeal genome sequence.
Ricardo Ciria, Blanca Taboada, Enrique Merino, Karel Estrada   +3 more
core   +1 more source

Induction and Enhancement of Bacteriocin Production (Review)

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Conventionally, bacteriocins are produced by microorganisms on complex substrates, released as semi‐purified preparations or crude fermentates. These bacteriocin‐containing products often exhibit unsatisfactory purity, low active‐substance content, and high production costs, limiting their commercialization and use. This review aims to analyze
Oksana Knysh, Artur Martynov
wiley   +1 more source

Identification of Putative Cellulose Synthase Gene xcsA2 Required for c‐di‐GMP‐Induced Biofilm Formation and Cell Aggregation in Synechococcus elongatus PCC 7942

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Synechococcus elongatus PCC 7942 is a widely used model cyanobacterium for diverse research fields and for bioproduction applications owing to its ease of cultivation, genetic modification, and ability to fix CO2. We found that a laboratory strain of S. elongatus PCC 7942 expressing the ydeH gene from E.
Chihiro C. Yamaguchi, Jiro F. Mori, Shinsuke Kutsuna, Robert A. Kanaly   +3 more
wiley   +1 more source