Results 101 to 110 of about 114,759 (195)

Iterative Genome Engineering Platform Enables Efficient Sucrose Biosynthesis From CO2 in Photosynthetic Synechococcus elongatus UTEX 2973

Plant Biotechnology Journal, EarlyView.
ABSTRACT The single crossover occurring via homologous recombination is a common phenomenon existing among microbes like Escherichia coli, Bacillus subtilis, Vibrio natriegens, Gluconobacter oxydans and most cyanobacteria species, threatening the stability of engineered strains and challenging iterative genetic engineering. Among them, we take the fast‐
Shubin Li, Tao Sun, Dailin Liu, Tong Zhang, Lei Chen, Weiwen Zhang   +5 more
wiley   +1 more source

RNA‐Guided Engineering of the Chloroplast Genome Enabled by Plastid‐Expressed Guide RNAs

Plant Biotechnology Journal, EarlyView.
ABSTRACT Our goal is to develop RNA‐guided engineering of the chloroplast genome using the CRISPR/Cas9 system. We designed chloroplast minigenes to obtain properly sized single guide RNAs (sgRNAs) in tobacco chloroplasts. The sgRNA 5′ end is defined by transcription from an rRNA operon promoter, and its 3′ end by processing a downstream tRNA (trnG) or ...
Malihe Mirzaee, Corinne Best, Evelyn V. Wachowski, Pal Maliga   +3 more
wiley   +1 more source

Evolution‐Inspired Engineering of Diterpene Biosynthesis via Chloroplast Genome Modification

Plant Biotechnology Journal, EarlyView.
ABSTRACT Terpenes constitute the largest and most structurally diverse class of plant secondary metabolites, with critical roles in plant‐environment interactions and broad industrial applications. Although nuclear genome engineering of terpene pathways has been extensively explored, chloroplast genome engineering remains largely undeveloped, with all ...
Alessandro Occhialini, Xinlu Chen, Samantha A. Miller, Mohammad Majdi, Ivette A. Fuentes Quispe, Gabriella King, Feng Chen   +6 more
wiley   +1 more source

Advances in large DNA fragment assembly for microbial cell factory engineering

Quantitative Biology, Volume 14, Issue 3, September 2026.
Abstract The efficient, rapid, and reliable assembly of DNA fragments is essential for advancing metabolic engineering and synthetic biology. With the rapid advancement of DNA synthesis and assembly technologies, the scale of DNA assembly has expanded from single genes to metabolic pathways and even genomes.
Yu Zhang, Wenqian Liu, Dongyuan Cheng, Jiazhang Lian, Xian Fu, Yue Shen   +5 more
wiley   +1 more source

Complete Chloroplast Genome of Indonesian Sugarcane (Saccharum ‘Bululawang’): Comparative, Structural, and Evolutionary Analysis of Photosystem I‐Encoding Genes

Journal of Sustainable Agriculture and Environment, Volume 5, Issue 3, September 2026.
ABSTRACT Sugarcane is one of the most important crops, providing the majority of global sugar production and serving as a major feedstock for bioenergy. Increasing demands for sustainable sugar production under climate and productivity constraint have intensified the need for molecular‐level characterization of elite cultivars.
Widhi Dyah Sawitri, Dwi Kusuma Wahyuni, Triono Bagus Saputro, Denianto Agung Wicaksono, Muhammad Rafi Fairuza, Tiara Putria Judith, Ganies Riza Aristya   +6 more
wiley   +1 more source

Removal of Photosystem I Subunit PsaE Leads to Photosynthetic Hydrogen Production in a Cyanobacterial PsaF‐HoxYH Fusion Strain

Plant, Cell &Environment, Volume 49, Issue 9, Page 6850-6858, September 2026.
ABSTRACT Photosynthetic hydrogen (photoH2) production is a promising approach for the utilisation of solar energy. Fusion of a hydrogenase (H2ase) to photosystem I (PSI) provides a direct light‐driven route for photoH2 generation. We established the lateral transmembrane subunit PsaF of PSI as a new fusion partner for the NiFe‐hydrogenase (HoxYH) in ...
Florian Paul, Nadine Strabel, Marko Boehm, Jens Appel, Kirstin Gutekunst   +4 more
wiley   +1 more source

Syndromic Convergence in Aquaculture Disease Management: A Critical Review of Climate‐Pathobiome Interactions, Diagnostic Frameworks, and Knowledge Gaps

Reviews in Aquaculture, Volume 18, Issue 4, September 2026.
Syndromic convergence (SyCo) reframes aquaculture disease as the synergistic product of climate‐driven stressors, pathobiome communities, and host immune compromise, not single pathogens. A five‐criterion scoring matrix, composite early warning index, and three‐layer intervention framework together operationalize this shift toward predictive ...
Ilhan Altinok
wiley   +1 more source

Actinomycin Derivatives: Structural Diversification and Biological Activities

ChemMedChem, Volume 21, Issue 15, 14 August 2026.
Actinomycins are chromopeptide antibiotics exhibiting remarkable structural diversity and broad bioactivity. This review traces their development from naturally occurring D‐, X‐, A‐, G‐, Y‐, and Z‐type analogs to precursor‐directed, synthetic, and semisynthetic derivatives.
Özge Can, Erdal Bedir
wiley   +1 more source

A Transcriptional Switch for Recombinant Protein Expression in Seed Plastids

Plant Biotechnology Journal, EarlyView.
Malihe Mirzaee, Evelyn V. Wachowski, Injy H. Elgamal, Pal Maliga   +3 more
wiley   +1 more source

Fragmentation of Vancoresmycin Reveals a Strong Dependence on Global Molecular Architecture for Antibacterial Activity

ChemMedChem, Volume 21, Issue 15, 14 August 2026.
Fragmentation of the natural product vancoresmycin reveals an essential role of global architecture for biological potency. Vancoresmycin is a structurally complex tetramic‐acid‐containing natural product with potent activity against Gram‐positive bacteria, yet its structure–activity relationships remain poorly defined.
Max Schönenbroicher, Florian Heppner, Maximilian Seul, Simon Morgenschweis, Aia Ali Abdelrahman, Eva Fahr, Niklas Holstein, Anna Müller, Dirk Menche   +8 more
wiley   +1 more source