Results 111 to 120 of about 493,288 (280)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Is Computing with Light All You Need? A Perspective on Codesign for Optical Artificial Intelligence and Scientific Computing

Advanced Intelligent Systems
Anything and everything can be solved with light—but under what contexts should one use optical computing and how should optical systems be designed for these tasks?
Prasad P. Iyer, Gaurang R. Bhatt, Saaketh Desai, Elliot J. Fuller, Corinne M. Teeter, François Léonard, Craig M. Vineyard   +6 more
doaj   +1 more source

Space-efficient optical computing with an integrated chip diffractive neural network. [PDF]

Nat Commun, 2022
Zhu HH, Zou J, Zhang H, Shi YZ, Luo SB, Wang N, Cai H, Wan LX, Wang B, Jiang XD, Thompson J, Luo XS, Zhou XH, Xiao LM, Huang W, Patrick L, Gu M, Kwek LC, Liu AQ.   +18 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Computer-free computational imaging: optical computing for seeing through random media. [PDF]

Light Sci Appl, 2022
Li Y, Tian L.
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Digital-analog hybrid matrix multiplication processor for optical neural networks

Nature Communications
Optical neural networks (ONNs) promise computing efficiency beyond microelectronics for modern artificial intelligence (AI). Current ONNs using analog matrix-vector multiplication (MVM) implementations are fundamentally limited in numerical precision due
Xiansong Meng, Deming Kong, Kwangwoong Kim, Qiuchi Li, Po Dong, Ingemar J. Cox, Christina Lioma, Hao Hu   +7 more
doaj   +1 more source

Recent advances in integrated optical directed logic operations for high performance optical computing: a review. [PDF]

Front Optoelectron, 2022
Qiu C, Xiao H, Wang L, Tian Y.
europepmc   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source