Results 91 to 100 of about 24,735 (218)

Spatial biology in cancer epigenetics

Molecular Oncology, EarlyView.
Spatial epigenomics combines molecular profiling with tissue architecture to reveal how gene regulation is organized within intact tissues. In cancer, these technologies uncover the mechanisms driving tumor heterogeneity and microenvironmental interactions, opening new opportunities for biomarker discovery and precision medicine.
Eva Crespo‐García, Manel Esteller
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Three-Dimension Calculation for the Scattering Problem for Non-Spherical Potential

Physics
The interaction of the 238U with a neutron is studied. Correct accounting for the non-spherical shape of the uranium nucleus is in focus. The optical potential is used as a model.
Pavel M. Krassovitskiy, Fedor M. Pen’kov   +1 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Systematic study of the propagation of uncertainties to transfer observables

Frontiers in Physics
A systematic study of parametric uncertainties in transfer reactions is performed using the recently developed uncertainty quantified global optical potential (KDUQ).
C. Hebborn, C. Hebborn, C. Hebborn, F. M. Nunes, F. M. Nunes   +4 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Non-destructive analysis of the optical effects of coloured grounds of paintings using flux-based statistical light scattering

JPhys Photonics
A ground is a layer applied to a painter’s canvas or panel to create an evenly absorbing, monochrome surface. Coloured ground layers can have a crucial visual impact on paintings. Many seventeenth-century paintings have been executed on a coloured ground.
Xiang Wang, Laura Levine, Andrei Anisimov, Maartje Stols-Witlox, Roger M Groves   +4 more
doaj   +1 more source

Optical Model And Cross Section Uncertainties

EPJ Web of Conferences, 2010
Distinct minima and maxima in the neutron total cross section uncertainties were observed in model calculations using spherical optical potential.
Dietrich F. S., Obložinský P., Herman M., Pigni M. T.   +3 more
doaj   +1 more source