Results 51 to 60 of about 108,344 (263)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

How Fast Is Your Body Motion? Determining a Sufficient Frame Rate for an Optical Motion Tracking System Using Passive Markers. [PDF]

open access: yesPLoS ONE, 2016
This paper addresses how to determine a sufficient frame (sampling) rate for an optical motion tracking system using passive reflective markers. When using passive markers for the optical motion tracking, avoiding identity confusion between the markers ...
Min-Ho Song, Rolf Inge Godøy
doaj   +1 more source

Search space reduction in optical tracking [PDF]

open access: yesProceedings of the workshop on Virtual environments 2003, 2003
In this paper we present a practical method for reducing the space when searching for point patterns in optically tracked virtual reality applications. The method uses a predictor to estimate the future position of a point. The key idea is to define a metric that determines the quality of the predictor, and use this metric to construct a 3D region ...
R. van Liere (Robert)   +1 more
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Neural fields for 3D tracking of anatomy and surgical instruments in monocular laparoscopic video clips

open access: yesHealthcare Technology Letters
Laparoscopic video tracking primarily focuses on two target types: surgical instruments and anatomy. The former could be used for skill assessment, while the latter is necessary for the projection of virtual overlays.
Beerend G. A. Gerats   +3 more
doaj   +1 more source

High-Precision Alignment Method for Electro-Optic Modulators via Combined Twyman-Green and Conoscopic Interferometry

open access: yesSensors
Electro-optic modulators (EOMs) are critical components in advanced optical systems, including quantum communications and high-resolution imaging, where precise alignment is essential for optimal performance.
Peng Zhang, Qi Lu
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

StraightTrack: Towards mixed reality navigation system for percutaneous K‐wire insertion

open access: yesHealthcare Technology Letters
In percutaneous pelvic trauma surgery, accurate placement of Kirschner wires (K‐wires) is crucial to ensure effective fracture fixation and avoid complications due to breaching the cortical bone along an unsuitable trajectory.
Han Zhang   +11 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

High-Order Temporal Context-Aware Aerial Tracking with Heterogeneous Visual Experts

open access: yesRemote Sensing
Visual tracking from the unmanned aerial vehicle (UAV) perspective has been at the core of many low-altitude remote sensing applications. Most of the aerial trackers follow “tracking-by-detection” paradigms or their temporal-context-embedded variants ...
Shichao Zhou   +4 more
doaj   +1 more source

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