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Real-Time Monitoring of Mass and Mechanical Properties in Single Cells and Multicellular Spheroids. [PDF]

open access: yesAdv Sci (Weinh)
Incaviglia I   +8 more
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Optical trapping and binding

Reports on Progress in Physics, 2013
The phenomenon of light's momentum was first observed in the laboratory at the beginning of the twentieth century, and its potential for manipulating microscopic particles was demonstrated by Ashkin some 70 years later. Since that initial demonstration, and the seminal 1986 paper where a single-beam gradient-force trap was realized, optical trapping ...
Richard W, Bowman, Miles J, Padgett
openaire   +2 more sources

Optical Trapping in Plant Cells

2013
Optical tweezers allow noninvasive manipulation of subcellular compartments to study their physical interactions and attachments. By measuring (delay of) displacements, (semi-)quantitative force measurements within a living cell can be performed. In this chapter, we provide practical tips for setting up such experiments paying special attention to the ...
Ketelaar, T.   +2 more
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Servo control of an optical trap

Applied Optics, 2007
A versatile optical trap has been constructed to control the position of trapped objects and ultimately to apply specified forces using feedback control. While the design, development, and use of optical traps has been extensive and feedback control has played a critical role in pushing the state of the art, few comprehensive examinations of feedback ...
Kurt D, Wulff   +2 more
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Optical Traps for Dark Excitons

Physical Review Letters, 2011
We propose a mechanism for optical trapping of dark excitons by linearly polarized unabsorbed standing waves, with a potential depth of the order of a few meV. Since this trapping, based on carrier exchanges with virtual excitons coupled to unabsorbed photons, equally acts on bright and dark states, Bose-Einstein condensation of excitons--which occurs ...
Combescot, Monique   +2 more
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Optical vortex trapping of particles

Optics Letters, 1996
We demonstrate three-dimensional trapping of low-index particles (20-microm-diameter hollow glass spheres in water) by using a single, strongly focused, stationary dark optical vortex laser beam. The holographically generated vortex, which is similar to a TEM(01)* mode beam, was also used to trap and form ring patterns of high-index particles.
K T, Gahagan, G A, Swartzlander
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Optical traps with geometric aberrations

Applied Optics, 2006
We assess the influence of geometric aberrations on the in-plane performance of optical traps by studying the dynamics of trapped colloidal spheres in deliberately distorted holographic optical tweezers. The lateral stiffness of the traps turns out to be insensitive to moderate amounts of coma, astigmatism, and spherical aberration.
Yael, Roichman   +3 more
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Optical trapping for analytical biotechnology

Current Opinion in Biotechnology, 2012
We describe the exciting advances of using optical trapping in the field of analytical biotechnology. This technique has opened up opportunities to manipulate biological particles at the single cell or even at subcellular levels which has allowed an insight into the physical and chemical mechanisms of many biological processes.
Praveen C, Ashok, Kishan, Dholakia
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Optical Trapping and Unfolding of RNA

2011
Optical tweezers have proven a useful tool for exploring the structure and function of individual molecules, such as proteins, DNA, and RNA. The ability to unfold and refold biological molecules has provided novel insights that complement and go beyond traditional biochemical and structural approaches.
Katherine H, White, Koen, Visscher
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Optical trapping of carbon nanotubes

Physica E: Low-dimensional Systems and Nanostructures, 2008
Optical trapping is a new tool for the manipulation and deposition of single wall carbon nanotube (SWNT) bundles. We present a study on optical trapping and manipulation of SWNT bundles in different environments, aimed at understanding the trapping mechanism. SWNTs are dispersed in water or organic solvents, and a wide range of both ionic and non-ionic
Maragò O M   +11 more
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